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Literature summary extracted from

  • Serra, J.L.; Llama, M.J.; Rowell, P.; Stewart, W.D.P.
    Purification and characterization of phosphoribulokinase from the N2-fixing cyanobacterium Anabaena cylindrica (1989), Plant Sci., 59, 1-9.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.19 thioredoxin activation by ditiothreitol-reduced thioredoxin, 3.3fold Anabaena cylindrica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.19 0.29
-
 D-ribulose 5-phosphate pH 7.5, 30°C Anabaena cylindrica
2.7.1.19 1.42
-
 ATP pH 7.5, 30°C Anabaena cylindrica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.19 Ca2+ no activity Anabaena cylindrica
2.7.1.19 Mg2+ required Anabaena cylindrica
2.7.1.19 Mn2+ no activity Anabaena cylindrica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.19 26000
-
 1 * 43000 + 1 * 26000, SDS-PAGE in presence of 2-mercaptoethanol Anabaena cylindrica
2.7.1.19 43000
-
 1 * 43000 + 1 * 26000, SDS-PAGE in presence of 2-mercaptoethanol Anabaena cylindrica
2.7.1.19 72000
-
 gel filtration Anabaena cylindrica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP Anabaena cylindrica
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Anabaena cylindrica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19
-
 Anabaena cylindrica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.19 1.95
-
-
 Anabaena cylindrica
2.7.1.19 15.17
-
 after activation by reduced thioredoxin Anabaena cylindrica

Storage Stability

EC Number Storage Stability Organism
2.7.1.19 -20°C, 50 mM Tris-HCl buffer, pH 8.0, 1 mM 2-mercaptoethanol Anabaena cylindrica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Anabaena cylindrica D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by CTP with reduced activity Anabaena cylindrica D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by UTP with reduced activity Anabaena cylindrica D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + ATP ATP can be substituted by GTP with reduced activity Anabaena cylindrica D-ribulose 1,5-diphosphate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.19 dimer 1 * 43000 + 1 * 26000, SDS-PAGE in presence of 2-mercaptoethanol Anabaena cylindrica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.19 8.6
-
-
 Anabaena cylindrica