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Literature summary extracted from

  • Lee, J.M.; Greenleaf, A.L.
    A protein kinase that phosphorylates the C-terminal repeat domain of the largest subunit of RNA polymerase II (1989), Proc. Natl. Acad. Sci. USA, 86, 3624-3628.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.23 0.00022
-
 CTD-containing fusion protein room temperature, pH 7.8 Saccharomyces cerevisiae
2.7.11.23 0.027
-
 ATP room temperature, pH 7.8 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.23 nucleus
-
 Drosophila sp. (in: flies) 5634
-
2.7.11.23 nucleus
-
 Homo sapiens 5634
-
2.7.11.23 nucleus
-
 Saccharomyces cerevisiae 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.23 Mg2+ requirement Drosophila sp. (in: flies)
2.7.11.23 Mg2+ requirement Homo sapiens
2.7.11.23 Mg2+ requirement Saccharomyces cerevisiae
2.7.11.23 Mg2+ above 1 mM Saccharomyces cerevisiae
2.7.11.23 additional information no activation by Ca2+ Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.23 32000
-
 tentatively alphabetagamma, 1 * 58000 + 1 * 38000 + 1 * 32000, SDS-PAGE Saccharomyces cerevisiae
2.7.11.23 38000
-
 tentatively alphabetagamma, 1 * 58000 + 1 * 38000 + 1 * 32000, SDS-PAGE Saccharomyces cerevisiae
2.7.11.23 58000
-
 tentatively alphabetagamma, 1 * 58000 + 1 * 38000 + 1 * 32000, SDS-PAGE Saccharomyces cerevisiae
2.7.11.23 120000
-
 glycerol density gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] Drosophila sp. (in: flies)
-
 ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] Homo sapiens
-
 ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] Saccharomyces cerevisiae
-
 ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] Drosophila sp. (in: flies) Kc
-
 ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.23 Drosophila sp. (in: flies)
-
 Kc
-
2.7.11.23 Drosophila sp. (in: flies) Kc
-
 Kc
-
2.7.11.23 Homo sapiens
-
-
-
2.7.11.23 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.23 partial Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.23 cell culture
-
 Homo sapiens
-
2.7.11.23 cell culture
-
 Saccharomyces cerevisiae
-
2.7.11.23 HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.23 65040
-
 after purification with Mono S column Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.23 ATP + CTD-containing fusion protein
-
 Saccharomyces cerevisiae ADP + phosphorylated CTD-containing fusion protein
-
 ?
2.7.11.23 ATP + CTD-containing fusion proteins
-
 Homo sapiens ADP + ?
-
 ?
2.7.11.23 ATP + CTD-containing fusion proteins
-
 Saccharomyces cerevisiae ADP + ?
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
-
 Drosophila sp. (in: flies) ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
-
 Homo sapiens ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
-
 Saccharomyces cerevisiae ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] no substrate is phosvitin Saccharomyces cerevisiae ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates not Tyr-residues Drosophila sp. (in: flies) ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates not Tyr-residues Homo sapiens ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates not Tyr-residues Saccharomyces cerevisiae ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates to a lesser extent Thr-residues Drosophila sp. (in: flies) ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates to a lesser extent Thr-residues Saccharomyces cerevisiae ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] kinase CTDK2 phosphorylates to a lesser extent Thr-residues Homo sapiens ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates predominantly Ser-residues Drosophila sp. (in: flies) ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates predominantly Ser-residues Homo sapiens ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates predominantly Ser-residues Saccharomyces cerevisiae ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
-
 Drosophila sp. (in: flies) Kc ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates not Tyr-residues Drosophila sp. (in: flies) Kc ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates to a lesser extent Thr-residues Drosophila sp. (in: flies) Kc ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
2.7.11.23 ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates predominantly Ser-residues Drosophila sp. (in: flies) Kc ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.23 trimer tentatively alphabetagamma, 1 * 58000 + 1 * 38000 + 1 * 32000, SDS-PAGE Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.23 additional information no activation by cAMP Saccharomyces cerevisiae
2.7.11.23 additional information no activation by calmodulin and cGMP Saccharomyces cerevisiae