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Literature summary extracted from

  • Carpenter, C.L.; Duckworth, B.C.; Auger, K.R.; Cohen, B.; Schaffhausen, B.S.; Cantley, L.C.
    Purification and characterization of phosphoinositide 3-kinase from rat liver (1990), J. Biol. Chem., 265, 19704-19711.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.137 cardiolipin
-
 Rattus norvegicus
2.7.1.137 lysophosphatidic acid
-
 Rattus norvegicus
2.7.1.137 phosphatidic acid
-
 Rattus norvegicus
2.7.1.137 phosphatidylcholine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.137 0.004
-
 phosphatidylinositol 4,5-bisphosphate
-
 Rattus norvegicus
2.7.1.137 0.009
-
 phosphatidylinositol 4-phosphate
-
 Rattus norvegicus
2.7.1.137 0.03
-
 ATP with phosphatidylinositol 4-phosphate or phosphatidylinositol 4,5-bisphosphate as cosubstrate Rattus norvegicus
2.7.1.137 0.06
-
 phosphatidylinositol
-
 Rattus norvegicus
2.7.1.137 0.06
-
 ATP with phosphatidylinositol as cosubstrate Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.137 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.137 Ca2+ no effect at physiological concentrations Rattus norvegicus
2.7.1.137 Mg2+ required Rattus norvegicus
2.7.1.137 Mg2+ maximal activation at 5 mM Rattus norvegicus
2.7.1.137 Mn2+ can partially replace Mg2+ in activation, about 10% of the activity with Mg2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.137 85000
-
 1 * 110000 + 1 * 85000, SDS-PAGE Rattus norvegicus
2.7.1.137 110000
-
 1 * 110000 + 1 * 85000, SDS-PAGE Rattus norvegicus
2.7.1.137 190000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.137 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.137
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.137 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.137 0.058
-
 production of phosphatidylinositol 3,4-diphosphate Rattus norvegicus
2.7.1.137 0.124
-
 production of phosphatidylinositol 3-phosphate Rattus norvegicus
2.7.1.137 0.214
-
 production of phosphatidylinositol 3,4,5-triphosphate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.137 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.1.137 ATP + 1-phosphatidylinositol
-
 Rattus norvegicus ADP + phosphatidylinositol 3-phosphate
-
 ?
2.7.1.137 ATP + phosphatidylinositol 4,5-bisphosphate
-
 Rattus norvegicus ADP + phosphatidylinositol 3,4,5-trisphosphate
-
 ?
2.7.1.137 ATP + phosphatidylinositol 4-phosphate
-
 Rattus norvegicus ADP + phosphatidylinositol 3,4-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.137 dimer 1 * 110000 + 1 * 85000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.137 7 8
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.137 6.5 9 pH 6.5: about 80% of maximal activity, pH 9.0: about 40% of maximal activity Rattus norvegicus