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Literature summary extracted from

  • Brzeska, H.; Young, R.; Tan, C.; Szczepanowska, J.; Korn, E.D.
    Calmodulin-binding and autoinhibitory domains of Acanthamoeba myosin I heavy chain kinase, a p21-activated kinase (PAK) (2001), J. Biol. Chem., 276, 47468-47473.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 phosphatidylserine
-
 Acanthamoeba castellanii
2.7.11.7 Rac reverses autoinhibition of the enzyme Acanthamoeba castellanii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.7 construction of His-tagged or FLAG-tagged N-terminal enzyme peptides by site directed mutagenesis for introduction of start and stop codons, expression in Escherichia coli BL21(DE3) Acanthamoeba castellanii
2.7.11.7 subcloning and expression in Escherichia coli, expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system Acanthamoeba castellanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.7 Ca2+/calmodulin calmodulin-binding site: amino acid residues 51-80; competes with phospholipids Acanthamoeba castellanii
2.7.11.7 EGTA
-
 Acanthamoeba castellanii
2.7.11.7 additional information autoinhibitory domains, overview, autoinhibition is reversed by Rac Acanthamoeba castellanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Ca2+ slightly activates unphosphorlyated enzyme Acanthamoeba castellanii
2.7.11.7 Mg2+
-
 Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin I heavy chain Acanthamoeba castellanii
-
 ADP + myosin I heavy chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Acanthamoeba castellanii Q93107 purified myosin I heavy chain kinase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 recombinant peptides of the enzymes N-terminus from Escherichia coli, His- or FLAG-tagged, and recombinant enzyme from Sf9 insect cells Acanthamoeba castellanii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.7 additional information
-
-
 Acanthamoeba castellanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + GRGRSSVYS synthetic peptide with a sequence corresponding to the phosphorylation site of myosin IC Acanthamoeba castellanii ADP + GRGRSS(-phosphate)VYS
-
 ?
2.7.11.7 ATP + myosin I heavy chain
-
 Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain substrate is heavy chain of myosin IC Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.7 MIHCK
-
 Acanthamoeba castellanii
2.7.11.7 More member of the p21-activated kinase family Acanthamoeba castellanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 20
-
 assay at Acanthamoeba castellanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7
-
 assay at Acanthamoeba castellanii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Acanthamoeba castellanii