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Literature summary extracted from

  • Rieker, J.P.; Swanljung-Collins, H.; Montibeller, J.; Collins, J.H.
    Purification and characterization of a calmodulin-dependent myosin heavy chain kinase from intestinal brush border (1987), J. Biol. Chem., 262, 15262-15268.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.7 freeze-thawing inactivates Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.7 EGTA
-
Gallus gallus
2.7.11.7 NaCl above 0.1 M Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Ca2+ requirement, Ca2+/calmodulin-dependent isozyme Gallus gallus
2.7.11.7 Mg2+ requirement Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.7 50000
-
10 * 50000, SDS-PAGE, asymmetric complex with an axial ratio calculated for prolate ellipsoid of 6.1 Gallus gallus
2.7.11.7 490000
-
gel filtration, sucrose density gradient centrifugation Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin heavy chain Gallus gallus
-
ADP + myosin heavy chain phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Gallus gallus
-
the Ca2+/calmodulin dependent heavy chain kinase has several properties in common with the family of type II calmodulin-dependent protein kinases
-
2.7.11.7 Gallus gallus
-
two myosin heavy-chain kinases: a Ca2+/calmodulin dependent and a Ca2+/calmodulin independent enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 Ca2+/calmodulin-dependent heavy chain kinase, 84fold Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.7 epithelium of the intestine, brush border cells Gallus gallus
-
2.7.11.7 small intestine epithelium Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.7 0.00063
-
purified enzyme Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.7.11.7 0°C, at least 1 month Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + chicken gizzard myosin light chain no activity Gallus gallus ADP + chicken gizzard myosin light chain phosphate
-
?
2.7.11.7 ATP + myosin heavy chain
-
Gallus gallus ADP + myosin heavy chain phosphate
-
?
2.7.11.7 ATP + myosin heavy chain substrate specificity Gallus gallus ADP + myosin heavy chain phosphate
-
?
2.7.11.7 ATP + myosin heavy chain phosphorylates threonine residues Gallus gallus ADP + myosin heavy chain phosphate
-
?
2.7.11.7 ATP + myosin heavy chain rabbit skeletal myosin heavy chains are phosphorylated Gallus gallus ADP + myosin heavy chain phosphate
-
?
2.7.11.7 ATP + myosin heavy chain 0.7 mol phosphate per mol of heavy chain Gallus gallus ADP + myosin heavy chain phosphate
-
?
2.7.11.7 ATP + myosin heavy chain best substrate: chicken intestine brush border myosin Gallus gallus ADP + myosin heavy chain phosphate
-
?
2.7.11.7 ATP + myosin heavy chain specific for myosin heavy chains, no phosphorylation of light chains Gallus gallus ADP + myosin heavy chain phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.11.7 decamer 10 * 50000, SDS-PAGE, asymmetric complex with an axial ratio calculated for prolate ellipsoid of 6.1 Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 8
-
-
Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.7 6 9 about half-maximal activity at pH 6.0, about 90% of maximal activity at pH 7.0 and pH 9.0 Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
Gallus gallus