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Literature summary extracted from

  • Côté, G.P.; Bukiejko, U.
    Purification and characterization of a myosin heavy chain kinase from Dictyostelium discoideum (1987), J. Biol. Chem., 262, 1065-1072.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 additional information no activation by cGMP Dictyostelium discoideum
2.7.11.7 additional information no activation by EGTA Dictyostelium discoideum
2.7.11.7 additional information no activation by calmodulin, cAMP Dictyostelium discoideum

General Stability

EC Number General Stability Organism
2.7.11.7 very unstable if exposed to low salt, i.e. 50 mM KCl or less, in the absence of sucrose Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.7 Ca2+/calmodulin no inhibition Dictyostelium discoideum
2.7.11.7 EGTA no inhibition Dictyostelium discoideum
2.7.11.7 KCl strong, 90% inhibition at 60 mM, complete inhibition at 100 mM Dictyostelium discoideum
2.7.11.7 additional information no inhibition by Ca2+; no inhibition by cAMP or cGMP Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.7 cytosol
-
 Dictyostelium discoideum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Ca2+ not required Dictyostelium discoideum
2.7.11.7 Mg2+ requirement Dictyostelium discoideum
2.7.11.7 Mg2+ absolutely requires 1-2 mM Mg2+ Dictyostelium discoideum
2.7.11.7 Mg2+ cannot be substituted by Ca2+ or Mn2+ Dictyostelium discoideum
2.7.11.7 additional information no activation by Mn2+ Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.7 130000
-
 x * 130000, SDS-PAGE Dictyostelium discoideum
2.7.11.7 700000
-
 above, gel filtration Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin I heavy chain Dictyostelium discoideum reaction in regulatory contractile activity in Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Dictyostelium discoideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 14000fold to near homogeneity Dictyostelium discoideum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.7 cell culture growth-phase cells Dictyostelium discoideum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.7 0.98
-
 purified enzyme Dictyostelium discoideum

Storage Stability

EC Number Storage Stability Organism
2.7.11.7 0°C, in 10 mM imidazole, pH 7, 100 mM KCl, 2 mM DTT, 60% sucrose, several weeks Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + casein no activity Dictyostelium discoideum ADP + casein phosphate
-
 ?
2.7.11.7 ATP + histone 2A low activity Dictyostelium discoideum ADP + histone 2A phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase intramolecular autophosphorylation Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase leads to activation of the phosphorylation activity towards myosin I Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain myosin light chains are no substrates Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain phosphorylates threonine residues Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain 1.9 mol phosphate per mol myosin Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain no substrates are human platelet myosin, ovalbumin and smooth muscle myosin from turkey Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain reaction in regulatory contractile activity in Dictyostelium discoideum Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + phosvitin low activity Dictyostelium discoideum ADP + phosvitin phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.7 ? x * 130000, SDS-PAGE Dictyostelium discoideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 25
-
 assay at Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7 7.5
-
 Dictyostelium discoideum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.7 6 8.5 about half-maximal activity at pH 6.0 Dictyostelium discoideum
2.7.11.7 6 8.5 about 50% activity at pH 6.0 Dictyostelium discoideum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.11.7 8
-
 and above, unstable Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Dictyostelium discoideum