EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.14 | rhodopsin | photolyzed rhodopsin stimulates | Bos taurus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.11.14 | expression of RK and mutants in COS-7 cells | Bos taurus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.11.14 | K491A | mutant is unable to phosphorylate acidic peptides, residue participates in substrate binding | Bos taurus |
2.7.11.14 | additional information | mutations at the autophosphorylation region affect the Km for ATP and change the initial site of phosphorylation on photolyzed rhodopsin, influence of mutations on the affinity for heparin-Sepharose | Bos taurus |
2.7.11.14 | S488A | autophosphorylation site mutant with increased activity for the phosphorylation of rhodopsin in the dark | Bos taurus |
2.7.11.14 | S488A | S488A/T489A double mutant with almost eliminated autophosphorylation and increased ability to phosphorylate rhodopsin in the dark | Bos taurus |
2.7.11.14 | S488A | autophosphorylation site mutant with 50% reduced autophosphorylation | Bos taurus |
2.7.11.14 | S488D | autophosphorylation site mutant with 50% reduced autophosphorylation and increased ability to phosphorylate rhodopsin in the dark | Bos taurus |
2.7.11.14 | S488D | S488D/T489D double mutant with almost eliminated autophosphorylation | Bos taurus |
2.7.11.14 | T489A | S488A/T489A double mutant with almost eliminated autophosphorylation and increased ability to phosphorylate rhodopsin in the dark | Bos taurus |
2.7.11.14 | T489A | autophosphorylation site mutant with 50% reduced autophosphorylation | Bos taurus |
2.7.11.14 | T489D | autophosphorylation site mutant with 50% reduced autophosphorylation | Bos taurus |
2.7.11.14 | T489D | S488D/T489D double mutant with almost eliminated autophosphorylation | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.14 | heparin | modest inhibition | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.11.14 | 0.002 | 0.004 | rhodopsin | pH 7.5, 30°C, wild-type RK and mutants | Bos taurus | |
2.7.11.14 | 0.005 | - |
ATP | pH 7.5, 30°C, S488D mutant | Bos taurus | |
2.7.11.14 | 0.007 | - |
ATP | pH 7.5, 30°C, wild-type RK | Bos taurus | |
2.7.11.14 | 0.012 | 0.015 | ATP | pH 7.5, 30°C, K491A or T489A mutant | Bos taurus | |
2.7.11.14 | 0.025 | - |
ATP | pH 7.5, 30°C, S488D/T489D double mutant | Bos taurus | |
2.7.11.14 | 0.14 | - |
ATP | pH 7.5, 30°C, S488A/T489A double mutant | Bos taurus | |
2.7.11.14 | 0.166 | - |
ATP | pH 7.5, 30°C, S488A mutant | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.11.14 | membrane | membrane-bound | Bos taurus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.14 | Mg2+ | - |
Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.14 | ATP + rhodopsin | Bos taurus | RK partially terminates the biochemical events that follow photon absorption | ADP + phosphorhodopsin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.14 | Bos taurus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.11.14 | phosphoprotein | major autophosphorylation sites: Ser-488, Thr-489, the autophosphorylation region of RK is involved in binding of ATP to the catalytic site, it may regulate selectivity of the site of phosphorylation and may influence the rate of RK dissociation from phosphorylated photolyzed rhodopsin, mechanism of RK regulation by autophosphorylation | Bos taurus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.11.14 | native RK, recombinant RK and mutants expressed in COS-7 cells | Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.11.14 | on ice, RK mutants, 2 days, stable | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.14 | ATP + peptide | acidic peptides, stimulated by photolyzed rhodopsin, K-491 of RK participates in substrate binding | Bos taurus | ADP + phosphopeptide | - |
? | |
2.7.11.14 | ATP + rhodopsin | the autophosphorylation region of RK is involved in binding of ATP to the catalytic site and may regulate selectivity of the site of phosphorylation | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.14 | ATP + rhodopsin | highly specific for photobleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.14 | ATP + rhodopsin | phosphorylation sites of photolyzed rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.14 | ATP + rhodopsin | domain structure | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.14 | ATP + rhodopsin | RK partially terminates the biochemical events that follow photon absorption | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.14 | additional information | not: apoprotein opsin | Bos taurus | ? | - |
? | |
2.7.11.14 | additional information | not: unbleached rhodopsin | Bos taurus | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 30 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 7.5 | - |
assay at | Bos taurus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.11.14 | 0.3 | - |
heparin | pH 7.5, 30°C | Bos taurus |