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Literature summary extracted from

  • Palczewski, K.; Buzylko, J.; Kaplan, M.W.; Polans, A.S.; Crabb, J.W.
    Mechanism of rhodopsin kinase activation (1991), J. Biol. Chem., 266, 12949-12955.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.14 mastoparan stimulates, mechanism Bos taurus
2.7.11.14 additional information various enzymatically truncated forms of photolyzed rhodopsin stimulate, light-dependent Bos taurus
2.7.11.14 rhodopsin activation, allosteric, the kinase binds to cytoplasmic loops of rhodopsin Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.14 additional information
-
 additional information Km values for truncated forms of rhodopsin Bos taurus
2.7.11.14 0.004 0.0044 rhodopsin pH 7.5, 30°C Bos taurus
2.7.11.14 7.1
-
 DDEASTTVSKTETSQVARRR pH 7.5, 30°C, peptide C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.14 membrane rod membranes Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+ requirement Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.11.14 ATP + rhodopsin = ADP + phosphorhodopsin mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 retina rod cell outer segment Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + DDEASTTVSKTETSQVARRR synthetic peptide C, very poor substrate Bos taurus ?
-
 ?
2.7.11.14 ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin only phosphorylates C-terminal sites of rhodopsin, role of the cytoplasmic loops and C-terminal region of rhodopsin in binding and activating enzyme, V-VI loop is crucial for kinase binding, truncated forms of rhodopsin as substrates Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin phosphorylates bovine rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 7.5
-
 assay at Bos taurus