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Literature summary extracted from

  • Leder, I.G.
    Hog kidney gluconokinase (1957), J. Biol. Chem., 225, 125-136.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.12 Cu2+ ATP protects Sus scrofa
2.7.1.12 Mg2+ at each level of ATP the optimal molar concentration of Mg2+ is one-half that of ATP, higher concentration inhibits Sus scrofa
2.7.1.12 additional information not: iodoacetate Sus scrofa
2.7.1.12 NaF not Sus scrofa
2.7.1.12 p-chloromercuribenzoate
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.12 Ca2+ can partially replace Mg2+ in activation Sus scrofa
2.7.1.12 Co2+ can partially replace Mg2+ in activation Sus scrofa
2.7.1.12 Mg2+ activates Sus scrofa
2.7.1.12 Mg2+ at each level of ATP the optimal molar concentration of Mg2+ is one-half that of ATP, higher concentration inhibits Sus scrofa
2.7.1.12 Mn2+ can partially replace Mg2+ in activation Sus scrofa
2.7.1.12 Zn2+ can partially replace Mg2+ in activation Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.12 ATP + D-gluconate Sus scrofa
-
 ADP + 6-phospho-D-gluconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.12 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.12 partial Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.12 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.12 0.64
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.1.12 -15°C, 10% loss of activity after 3 weeks Sus scrofa
2.7.1.12 3°C, 50% loss of activity after 3 weeks Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.12 ATP + D-gluconate
-
 Sus scrofa ADP + 6-phospho-D-gluconate
-
 ?
2.7.1.12 ATP + D-gluconate strict specificity for D-gluconate Sus scrofa ADP + 6-phospho-D-gluconate
-
 ?
2.7.1.12 GTP + D-gluconate 25% of the activity with ATP Sus scrofa GDP + 6-phospho-D-gluconate
-
 ?
2.7.1.12 ITP + D-gluconate 60% of the activity with ATP Sus scrofa IDP + 6-phospho-D-gluconate
-
 ?
2.7.1.12 UTP + D-gluconate 130% of the activity with ATP Sus scrofa UDP + 6-phospho-D-gluconate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.12 34
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.12 6.2
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.12 5.1 8.4 pH 5.1: about 25% of activity maximum, pH 8.4: about 50% of activity maximum Sus scrofa