BRENDA - Enzyme Database

Inhibition of wheat embryo calcium-dependent protein kinase and avian myosin light chain kinase by flavonoids and related compounds

Jinsart, W.; Ternai, B.; Polya, G.M.; Biol. Chem. Hoppe-Seyler 372, 819-827 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.11.18
(+)-catechin
IC50: 0.44 mM
Gallus gallus
2.7.11.18
(-)-epicatechin
IC50: 0.32 mM
Gallus gallus
2.7.11.18
2,2'-Dihydroxychalcone
-
Gallus gallus
2.7.11.18
3',4',5'-tri-O-methyltricetin
-
Gallus gallus
2.7.11.18
3',4'-dihydroxyflavone
IC50: 0.262 mM
Gallus gallus
2.7.11.18
3,3',4'-trihydroxyflavone
IC50: 0.001 mM
Gallus gallus
2.7.11.18
5,4'-dihydroxyflavone
IC50: 0.024 mM
Gallus gallus
2.7.11.18
5,7-dihydroxyflavone
IC50: 0.043 mM
Gallus gallus
2.7.11.18
7,8,3',4'-tetrahydroxyflavone
IC50: 0.02 mM
Gallus gallus
2.7.11.18
7-O-methylapigenin
-
Gallus gallus
2.7.11.18
apigenin
IC50: 0.023 mM
Gallus gallus
2.7.11.18
chalcone
-
Gallus gallus
2.7.11.18
dihydroapigenin
IC50: 0.17 mM
Gallus gallus
2.7.11.18
dihydrofisetin
IC50: 0.18 mM
Gallus gallus
2.7.11.18
dihydroluteolin
-
Gallus gallus
2.7.11.18
dihydroquercetin
IC50: 0.08 mM
Gallus gallus
2.7.11.18
fisetin
IC50: 0.005 mM
Gallus gallus
2.7.11.18
galangin
IC50: 0.02 mM
Gallus gallus
2.7.11.18
gossypol
-
Gallus gallus
2.7.11.18
hesperidin
-
Gallus gallus
2.7.11.18
isoliquiritigenin
-
Gallus gallus
2.7.11.18
kaempferid
IC50: 0.008 mM
Gallus gallus
2.7.11.18
kaempferol
IC50: 0.004 mM
Gallus gallus
2.7.11.18
luteolin
IC50: 0.026 mM
Gallus gallus
2.7.11.18
additional information
no inhibition by epicatechin, pseudobabtisin, 4-dimethylaminobenzaldehyde
Gallus gallus
2.7.11.18
morin
IC50: 0.028 mM
Gallus gallus
2.7.11.18
myricetin
IC50: 0.006 mM
Gallus gallus
2.7.11.18
Okanin
-
Gallus gallus
2.7.11.18
pseudobaptisin
-
Gallus gallus
2.7.11.18
quercetagetin
IC50: 0.026 mM
Gallus gallus
2.7.11.18
quercetin
IC50: 0.006 mM
Gallus gallus
2.7.11.18
quercetrin
IC50: 0.137 mM
Gallus gallus
2.7.11.18
rutin
IC50: 0.32 mM
Gallus gallus
2.7.11.18
tricetin
IC50: 0.012 mM
Gallus gallus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.11.18
Ca2+
-
Gallus gallus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.11.18
ATP + myosin light chain
Gallus gallus
-
ADP + myosin light chain phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.11.18
Gallus gallus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.11.18
gizzard
-
Gallus gallus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.11.18
ATP + myosin light chain
-
640639
Gallus gallus
ADP + myosin light chain phosphate
-
-
-
?
2.7.11.18
ATP + myosin light chain
-
640639
Gallus gallus
ADP + myosin light chain phosphate
-
640639
Gallus gallus
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.11.18
30
-
assay at
Gallus gallus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.11.18
Calmodulin
requirement
Gallus gallus
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.11.18
0.001
-
IC50: 0.001 mM
Gallus gallus
3,3',4'-trihydroxyflavone
2.7.11.18
0.004
-
IC50: 0.004 mM
Gallus gallus
kaempferol
2.7.11.18
0.005
-
IC50: 0.005 mM
Gallus gallus
fisetin
2.7.11.18
0.006
-
IC50: 0.006 mM
Gallus gallus
myricetin
2.7.11.18
0.006
-
IC50: 0.006 mM
Gallus gallus
quercetin
2.7.11.18
0.008
-
IC50: 0.008 mM
Gallus gallus
kaempferid
2.7.11.18
0.012
-
IC50: 0.012 mM
Gallus gallus
tricetin
2.7.11.18
0.02
-
IC50: 0.02 mM
Gallus gallus
7,8,3',4'-tetrahydroxyflavone
2.7.11.18
0.02
-
IC50: 0.02 mM
Gallus gallus
galangin
2.7.11.18
0.023
-
IC50: 0.023 mM
Gallus gallus
apigenin
2.7.11.18
0.024
-
IC50: 0.024 mM
Gallus gallus
5,4'-dihydroxyflavone
2.7.11.18
0.026
-
IC50: 0.026 mM
Gallus gallus
luteolin
2.7.11.18
0.026
-
IC50: 0.026 mM
Gallus gallus
quercetagetin
2.7.11.18
0.028
-
IC50: 0.028 mM
Gallus gallus
morin
2.7.11.18
0.043
-
IC50: 0.043 mM
Gallus gallus
5,7-dihydroxyflavone
2.7.11.18
0.08
-
IC50: 0.08 mM
Gallus gallus
dihydroquercetin
2.7.11.18
0.137
-
IC50: 0.137 mM
Gallus gallus
quercetrin
2.7.11.18
0.17
-
IC50: 0.17 mM
Gallus gallus
dihydroapigenin
2.7.11.18
0.18
-
IC50: 0.18 mM
Gallus gallus
dihydrofisetin
2.7.11.18
0.262
-
IC50: 0.262 mM
Gallus gallus
3',4'-dihydroxyflavone
2.7.11.18
0.32
-
IC50: 0.32 mM
Gallus gallus
(-)-epicatechin
2.7.11.18
0.32
-
IC50: 0.32 mM
Gallus gallus
rutin
2.7.11.18
0.44
-
IC50: 0.44 mM
Gallus gallus
(+)-catechin
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.11.18
Calmodulin
requirement
Gallus gallus
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.11.18
0.001
-
IC50: 0.001 mM
Gallus gallus
3,3',4'-trihydroxyflavone
2.7.11.18
0.004
-
IC50: 0.004 mM
Gallus gallus
kaempferol
2.7.11.18
0.005
-
IC50: 0.005 mM
Gallus gallus
fisetin
2.7.11.18
0.006
-
IC50: 0.006 mM
Gallus gallus
myricetin
2.7.11.18
0.006
-
IC50: 0.006 mM
Gallus gallus
quercetin
2.7.11.18
0.008
-
IC50: 0.008 mM
Gallus gallus
kaempferid
2.7.11.18
0.012
-
IC50: 0.012 mM
Gallus gallus
tricetin
2.7.11.18
0.02
-
IC50: 0.02 mM
Gallus gallus
7,8,3',4'-tetrahydroxyflavone
2.7.11.18
0.02
-
IC50: 0.02 mM
Gallus gallus
galangin
2.7.11.18
0.023
-
IC50: 0.023 mM
Gallus gallus
apigenin
2.7.11.18
0.024
-
IC50: 0.024 mM
Gallus gallus
5,4'-dihydroxyflavone
2.7.11.18
0.026
-
IC50: 0.026 mM
Gallus gallus
luteolin
2.7.11.18
0.026
-
IC50: 0.026 mM
Gallus gallus
quercetagetin
2.7.11.18
0.028
-
IC50: 0.028 mM
Gallus gallus
morin
2.7.11.18
0.043
-
IC50: 0.043 mM
Gallus gallus
5,7-dihydroxyflavone
2.7.11.18
0.08
-
IC50: 0.08 mM
Gallus gallus
dihydroquercetin
2.7.11.18
0.137
-
IC50: 0.137 mM
Gallus gallus
quercetrin
2.7.11.18
0.17
-
IC50: 0.17 mM
Gallus gallus
dihydroapigenin
2.7.11.18
0.18
-
IC50: 0.18 mM
Gallus gallus
dihydrofisetin
2.7.11.18
0.262
-
IC50: 0.262 mM
Gallus gallus
3',4'-dihydroxyflavone
2.7.11.18
0.32
-
IC50: 0.32 mM
Gallus gallus
(-)-epicatechin
2.7.11.18
0.32
-
IC50: 0.32 mM
Gallus gallus
rutin
2.7.11.18
0.44
-
IC50: 0.44 mM
Gallus gallus
(+)-catechin
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.11.18
(+)-catechin
IC50: 0.44 mM
Gallus gallus
2.7.11.18
(-)-epicatechin
IC50: 0.32 mM
Gallus gallus
2.7.11.18
2,2'-Dihydroxychalcone
-
Gallus gallus
2.7.11.18
3',4',5'-tri-O-methyltricetin
-
Gallus gallus
2.7.11.18
3',4'-dihydroxyflavone
IC50: 0.262 mM
Gallus gallus
2.7.11.18
3,3',4'-trihydroxyflavone
IC50: 0.001 mM
Gallus gallus
2.7.11.18
5,4'-dihydroxyflavone
IC50: 0.024 mM
Gallus gallus
2.7.11.18
5,7-dihydroxyflavone
IC50: 0.043 mM
Gallus gallus
2.7.11.18
7,8,3',4'-tetrahydroxyflavone
IC50: 0.02 mM
Gallus gallus
2.7.11.18
7-O-methylapigenin
-
Gallus gallus
2.7.11.18
apigenin
IC50: 0.023 mM
Gallus gallus
2.7.11.18
chalcone
-
Gallus gallus
2.7.11.18
dihydroapigenin
IC50: 0.17 mM
Gallus gallus
2.7.11.18
dihydrofisetin
IC50: 0.18 mM
Gallus gallus
2.7.11.18
dihydroluteolin
-
Gallus gallus
2.7.11.18
dihydroquercetin
IC50: 0.08 mM
Gallus gallus
2.7.11.18
fisetin
IC50: 0.005 mM
Gallus gallus
2.7.11.18
galangin
IC50: 0.02 mM
Gallus gallus
2.7.11.18
gossypol
-
Gallus gallus
2.7.11.18
hesperidin
-
Gallus gallus
2.7.11.18
isoliquiritigenin
-
Gallus gallus
2.7.11.18
kaempferid
IC50: 0.008 mM
Gallus gallus
2.7.11.18
kaempferol
IC50: 0.004 mM
Gallus gallus
2.7.11.18
luteolin
IC50: 0.026 mM
Gallus gallus
2.7.11.18
additional information
no inhibition by epicatechin, pseudobabtisin, 4-dimethylaminobenzaldehyde
Gallus gallus
2.7.11.18
morin
IC50: 0.028 mM
Gallus gallus
2.7.11.18
myricetin
IC50: 0.006 mM
Gallus gallus
2.7.11.18
Okanin
-
Gallus gallus
2.7.11.18
pseudobaptisin
-
Gallus gallus
2.7.11.18
quercetagetin
IC50: 0.026 mM
Gallus gallus
2.7.11.18
quercetin
IC50: 0.006 mM
Gallus gallus
2.7.11.18
quercetrin
IC50: 0.137 mM
Gallus gallus
2.7.11.18
rutin
IC50: 0.32 mM
Gallus gallus
2.7.11.18
tricetin
IC50: 0.012 mM
Gallus gallus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.11.18
Ca2+
-
Gallus gallus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.11.18
ATP + myosin light chain
Gallus gallus
-
ADP + myosin light chain phosphate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.11.18
gizzard
-
Gallus gallus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.11.18
ATP + myosin light chain
-
640639
Gallus gallus
ADP + myosin light chain phosphate
-
-
-
?
2.7.11.18
ATP + myosin light chain
-
640639
Gallus gallus
ADP + myosin light chain phosphate
-
640639
Gallus gallus
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.11.18
30
-
assay at
Gallus gallus