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Literature summary extracted from

  • Serventi, I.M.; Coffee, C.J.
    Characterization of myosin light-chain kinase from bovine adrenal medulla (1986), Arch. Biochem. Biophys., 245, 379-388.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 additional information no activation by Ca2+ plus beta-lactoglobulin, cytochrome c, troponin C or parvalbumin Bos taurus
2.7.11.18 additional information no activation by cAMP Bos taurus

General Stability

EC Number General Stability Organism
2.7.11.18 protease inhibitors with broad specificity and glycerol stabilize during initial purification, unstable to further purification Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 Ca2+ at higher free concentrations, 0.4-3 mM, independent of Mg2+ or pH-value Bos taurus
2.7.11.18 EGTA
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 0.02 0.027 turkey gizzard myosin light chain 30°C, pH 7.6, ATP Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ inhibits at higher free concentrations Bos taurus
2.7.11.18 Ca2+ requirement, only in combination with calmodulin Bos taurus
2.7.11.18 Ca2+ aged enzyme loses Ca2+/calmodulin sensitivity by proteolysis Bos taurus
2.7.11.18 Mg2+
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 150000
-
 gel filtration and sedimentation studies Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + myosin light chain Bos taurus involved in regulation of actin-myosin contractile activity in adrenal medulla ADP + myosin light chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 adrenal medulla medulla Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of skeletal muscle Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of adrenal medullary myosin Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain 1 mol phosphate per mol light chain in skeletal muscle Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain specific for 20 kDa light chain Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain not 16 kDa light chain Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is phosphorylase b Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain involved in regulation of actin-myosin contractile activity in adrenal medulla Bos taurus ADP + myosin light chain phosphate
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 Calmodulin requirement Bos taurus
2.7.11.18 Calmodulin aged enzyme loses Ca2+/calmodulin sensitivity by proteolysis Bos taurus