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Literature summary extracted from

  • Hathaway, D.R.; Adelstein, R.S.
    Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for activity (1979), Proc. Natl. Acad. Sci. USA, 76, 1653-1657.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 EGTA
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 0.018
-
 myosin light chain 23-25°C, pH 7.5 Homo sapiens
2.7.11.18 0.121
-
 ATP 23-25°C, pH 7.5 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.18 actomyosin associated Homo sapiens 42641
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 105000
-
 x * 105000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + myosin light chain Homo sapiens
-
 ADP + myosin light chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18 partial, affinity chromatography on calmodulin-Sepharose Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 platelet
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.18 3.1
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + myosin light chain
-
 Homo sapiens ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrates are myosin heavy chain and phosvitin Homo sapiens ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is protamine Homo sapiens ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is histone 2-A Homo sapiens ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is casein Homo sapiens ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of smooth muscle Homo sapiens ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain specific for 20 kDa light chain Homo sapiens ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is histone V-S Homo sapiens ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is phosphorylase b Homo sapiens ADP + myosin light chain phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 ? x * 105000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.18 23 25 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.18 5.17
-
 myosin light chain 23-25°C, pH 7.5 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 Calmodulin requirement Homo sapiens