Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pires, E.M.V.; Perry, S.V.
    Purification and properties of myosin light-chain kinase from fast skeletal muscle (1977), Biochem. J., 167, 137-146.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 additional information no activation by cAMP Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 ATP free form, strong, not in the presence of excess Mg2+ Oryctolagus cuniculus
2.7.11.18 EGTA strong Oryctolagus cuniculus
2.7.11.18 Increasing ionic strength up to 0.4 M NaCl, weak Oryctolagus cuniculus
2.7.11.18 additional information no inhibition by 3',5'-cAMP; no inhibition by AMP Oryctolagus cuniculus
2.7.11.18 phosphate up to 0.1 M, weak Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 0.1 0.2 [rabbit white skeletal muscle myosin P-light chain] 27°C, pH 7.6 Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.18 sarcoplasm
-
 Oryctolagus cuniculus 16528
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ effect depends on Mg2+ concentration Oryctolagus cuniculus
2.7.11.18 Mg2+ requirement, varies with ATP-concentration, MgATP2- is the active substrate Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 77000
-
 gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + myosin light chain Oryctolagus cuniculus
-
 ADP + myosin light chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Oryctolagus cuniculus
-
 new Zealand white
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 skeletal muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.18 25
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + myosin light chain
-
 Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of skeletal muscle Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of cardiac muscle Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain ITP, GTP, CTP or UTP cannot replace ATP Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain not specific for 22 kDa and 15 kDa light chain Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is casein Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrates are 15 kDa and 22 kDa light-chain or heavy-chain fractions of myosin from white skeletal muscle Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is troponin Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain highly specific for regulatory or P-light-chain Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain highly specific for 18 kDa light chain Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of smooth muscle Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is molluscan adductor myosin Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain specific for 20 kDa light chain Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is phosphorylase b Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is histone III-S from thymus Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + [rabbit white skeletal muscle myosin P-light chain]
-
 Oryctolagus cuniculus ADP + [rabbit white skeletal muscle myosin P-light chain] phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 monomer 1 * 77000, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.18 25
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.18 6.5
-
-
 Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.18 5.7 8.2 about half-maximal activity at pH 5.7 and 8.2, with a small shoulder of 77% of maximal activity at 7-7.5 Oryctolagus cuniculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.11.18 5
-
 rapid inactivation below Oryctolagus cuniculus
2.7.11.18 6.3 8 stable in 10% sucrose Oryctolagus cuniculus