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Literature summary extracted from

  • Oudot, C.; Jaquinod, M.; Cortay, J.C.; Cozzone, A.J.; Jault, J.M.
    The isocitrate dehydrogenase kinase/phosphatase from Escherichia coli is highly sensitive to in-vitro oxidative conditions role of cysteine67 and cysteine108 in the formation of a disulfide-bonded homodimer (1999), Eur. J. Biochem., 262, 224-229.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.5 recombinant wild-type IDHK/P on overproducing plasmid pJCD4, expressed in Escherichia coli JM109 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.5 5,5'-dithio-bis(2-nitrobenzoic acid)
-
 Escherichia coli
2.7.11.5 cupric 1,10 phenanthrolinate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.5 66000
-
 2 * 66000, homodimer, SDS-PAGE Escherichia coli
2.7.11.5 68800
-
 2 * 68800, homodimer, theoretical molecular mass Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.5 ATP + [isocitrate dehydrogenase (NADP+)] Escherichia coli controls the oxidative metabolism, exibits a high intrinsic ATPase activity ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 r
2.7.11.5 ATP + [isocitrate dehydrogenase (NADP+)] Escherichia coli JM109 controls the oxidative metabolism, exibits a high intrinsic ATPase activity ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.5 Escherichia coli
-
 JM109
-
2.7.11.5 Escherichia coli JM109
-
 JM109
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.5
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.5 ATP + [isocitrate dehydrogenase (NADP+)]
-
 Escherichia coli ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 r
2.7.11.5 ATP + [isocitrate dehydrogenase (NADP+)] controls the oxidative metabolism, exibits a high intrinsic ATPase activity Escherichia coli ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 r
2.7.11.5 ATP + [isocitrate dehydrogenase (NADP+)]
-
 Escherichia coli JM109 ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 r
2.7.11.5 ATP + [isocitrate dehydrogenase (NADP+)] controls the oxidative metabolism, exibits a high intrinsic ATPase activity Escherichia coli JM109 ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.11.5 dimer 2 * 66000, homodimer, SDS-PAGE Escherichia coli
2.7.11.5 dimer 2 * 68800, homodimer, theoretical molecular mass Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.11.5 IDHK/P
-
 Escherichia coli
2.7.11.5 isocitrate dehydrogenase kinase/phosphatase
-
 Escherichia coli