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Literature summary extracted from

  • Grivell, A.R.; Jackson, J.F.
    Thymidine phosphotransferase and nucleotide phosphohydrolase of the fern Asplenium nidus. General properties and inhibition by adenosine 3:5-cyclic monophosphate (1976), Biochem. J., 155, 571-581.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.1.114 bovine serum albumin stabilizes Asplenium nidus
2.7.1.114 not stabilized by AMP or thymidine Asplenium nidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.114 1,10-phenanthroline
-
 Asplenium nidus
2.7.1.114 3',5'-cAMP
-
 Asplenium nidus
2.7.1.114 adenosine
-
 Asplenium nidus
2.7.1.114 CoCl2
-
 Asplenium nidus
2.7.1.114 MnCl2
-
 Asplenium nidus
2.7.1.114 NiCl2
-
 Asplenium nidus
2.7.1.114 uridine
-
 Asplenium nidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.114 0.11
-
 AMP 38°C, pH 7.5 Asplenium nidus
2.7.1.114 0.84 0.88 thymidine 38°C, pH 7.5 Asplenium nidus
2.7.1.114 1.28
-
 adenosine 38°C, pH 7.5 Asplenium nidus
2.7.1.114 4.71
-
 uridine 38°C, pH 7.5 Asplenium nidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.114 AMP + thymidine Hordeum vulgare
-
 adenosine + dTMP
-
 ?
2.7.1.114 AMP + thymidine Medicago sativa
-
 adenosine + dTMP
-
 ?
2.7.1.114 AMP + thymidine Helianthus tuberosus
-
 adenosine + dTMP
-
 ?
2.7.1.114 AMP + thymidine Asplenium nidus
-
 adenosine + dTMP
-
 ?
2.7.1.114 AMP + thymidine Epidendrum hybrid
-
 adenosine + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.114 Asplenium nidus
-
 bird's nest fern
-
2.7.1.114 Epidendrum hybrid
-
 crucifix orchid
-
2.7.1.114 Helianthus tuberosus
-
 Jerusalem artichoke
-
2.7.1.114 Hordeum vulgare
-
 barley
-
2.7.1.114 Medicago sativa
-
 lucerne
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.114 partial Asplenium nidus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.114 leaf
-
 Helianthus tuberosus
-
2.7.1.114 leaf
-
 Asplenium nidus
-
2.7.1.114 leaf
-
 Epidendrum hybrid
-
2.7.1.114 pollen
-
 Medicago sativa
-
2.7.1.114 root
-
 Hordeum vulgare
-
2.7.1.114 stem
-
 Helianthus tuberosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.114 0.002
-
 leaf Medicago sativa
2.7.1.114 0.01
-
 stem Helianthus tuberosus
2.7.1.114 0.05
-
 leaf Epidendrum hybrid
2.7.1.114 0.05
-
 roots Hordeum vulgare
2.7.1.114 0.06
-
 tops Hordeum vulgare
2.7.1.114 0.07
-
 senescent leaf Asplenium nidus
2.7.1.114 0.29
-
 mature leaf Helianthus tuberosus
2.7.1.114 0.47
-
 young and expanded leaf Helianthus tuberosus
2.7.1.114 0.84
-
 mature leaf Asplenium nidus
2.7.1.114 0.91
-
 young mature leaf Asplenium nidus
2.7.1.114 1.09
-
 immature leaf Asplenium nidus
2.7.1.114 1.64
-
 young bud Asplenium nidus

Storage Stability

EC Number Storage Stability Organism
2.7.1.114 0°C, 6 months, 20% loss of activity Asplenium nidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.114 AMP + 5-bromodeoxyuridine
-
 Asplenium nidus adenosine + 5-bromodeoxyuridine 5'-phosphate
-
 ?
2.7.1.114 AMP + adenosine
-
 Asplenium nidus adenosine + AMP
-
 ?
2.7.1.114 AMP + cytidine
-
 Asplenium nidus adenosine + cytidine 5'-phosphate
-
 ?
2.7.1.114 AMP + deoxyuridine
-
 Asplenium nidus adenosine + deoxyuridine 5'-phosphate
-
 ?
2.7.1.114 AMP + guanosine
-
 Asplenium nidus adenosine + guanosine 5'-phosphate
-
 ?
2.7.1.114 AMP + thymidine
-
 Hordeum vulgare adenosine + dTMP
-
 ?
2.7.1.114 AMP + thymidine
-
 Medicago sativa adenosine + dTMP
-
 ?
2.7.1.114 AMP + thymidine
-
 Helianthus tuberosus adenosine + dTMP
-
 ?
2.7.1.114 AMP + thymidine
-
 Asplenium nidus adenosine + dTMP
-
 ?
2.7.1.114 AMP + thymidine
-
 Epidendrum hybrid adenosine + dTMP
-
 ?
2.7.1.114 AMP + thymidine AMP can be substituted by GMP, dAMP, CMP, UMP, dUMP, dTMP Asplenium nidus adenosine + dTMP
-
 ?
2.7.1.114 AMP + uridine
-
 Asplenium nidus adenosine + uridine 5'-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.114 37
-
 60 min, 95% loss of activity Asplenium nidus
2.7.1.114 63
-
 5 min, complete inactivation Asplenium nidus