BRENDA - Enzyme Database

Partial purification and characterization of deoxyguanosine kinase from pig skin

Green, F.J.; Lewis, R.A.; Biochem. J. 183, 547-553 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.113
AMP
weak inhibition
Sus scrofa
2.7.1.113
ATP
weak inhibition
Sus scrofa
2.7.1.113
CMP
weak inhibition
Sus scrofa
2.7.1.113
dATP
0.1 mM, 62% inhibition
Sus scrofa
2.7.1.113
dCMP
weak inhibition
Sus scrofa
2.7.1.113
dCTP
0.1 mM, 48% inhibition
Sus scrofa
2.7.1.113
deoxyadenosine
weak inhibition
Sus scrofa
2.7.1.113
dGMP
0.1 mM, 44% inhibition
Sus scrofa
2.7.1.113
dGTP
0.1 mM, complete inhibition
Sus scrofa
2.7.1.113
dTMP
weak inhibition
Sus scrofa
2.7.1.113
dTTP
0.1 mM, 30% inhibition
Sus scrofa
2.7.1.113
GMP
weak inhibition
Sus scrofa
2.7.1.113
GTP
0.1 mM, 33% inhibition
Sus scrofa
2.7.1.113
additional information
not inhibited by dAMP
Sus scrofa
2.7.1.113
UMP
0.1 mM, 24% inhibition
Sus scrofa
2.7.1.113
UTP
0.1 mM, 30% inhibition
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.113
0.00032
-
deoxyguanosine
pH 8.0, 37°C
Sus scrofa
2.7.1.113
3.3
-
MgATP2-
pH 8.0, 37°C
Sus scrofa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.113
Ca2+
67% of activity with Mg2+; activation
Sus scrofa
2.7.1.113
Fe2+
slight activation
Sus scrofa
2.7.1.113
Mg2+
required for activity
Sus scrofa
2.7.1.113
Mn2+
92% of activity with Mg2+
Sus scrofa
2.7.1.113
additional information
divalent cations required, not activated by Zn2+
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.113
58500
-
gel filtration
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.113
Sus scrofa
-
piglet
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.113
pH 5.5, ammonium sulfate, DEAE-Sephadex
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.113
skin
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.113
0.0000338
-
-
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.113
ATP + deoxyguanosine
highly specific for phospho group acceptor
640552
Sus scrofa
ADP + dGMP
-
640552
Sus scrofa
?
2.7.1.113
ATP + deoxyguanosine
ATP twice as effective as CTP or GTP and 4 times as effective as UTP
640552
Sus scrofa
ADP + dGMP
-
640552
Sus scrofa
?
2.7.1.113
CTP + deoxyguanosine
50% of activity with ATP
640552
Sus scrofa
CDP + dGMP
-
-
-
?
2.7.1.113
GTP + deoxyguanosine
50% of activity with ATP
640552
Sus scrofa
GDP + dGMP
-
640552
Sus scrofa
?
2.7.1.113
UTP + deoxyguanosine
25% of activity with ATP
640552
Sus scrofa
UDP + dGMP
-
640552
Sus scrofa
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.113
37
-
-
Sus scrofa
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.1.113
30
53
17% loss of activity at 53°C, 50% loss of activity at 30°C and 53°C
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.113
8.5
-
-
Sus scrofa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.113
5
9
approx. 55% of maximal activity at pH 5.5 and pH 9, plateau with approx. 85% of maximal activity at pH 6.0-7.5
Sus scrofa
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.113
0.021
-
dGTP
pH 8.0, 37°C
Sus scrofa
2.7.1.113
0.076
-
dGMP
pH 8.0, 37°C
Sus scrofa
2.7.1.113
0.34
-
deoxyadenosine
pH 8.0, 37°C
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.113
AMP
weak inhibition
Sus scrofa
2.7.1.113
ATP
weak inhibition
Sus scrofa
2.7.1.113
CMP
weak inhibition
Sus scrofa
2.7.1.113
dATP
0.1 mM, 62% inhibition
Sus scrofa
2.7.1.113
dCMP
weak inhibition
Sus scrofa
2.7.1.113
dCTP
0.1 mM, 48% inhibition
Sus scrofa
2.7.1.113
deoxyadenosine
weak inhibition
Sus scrofa
2.7.1.113
dGMP
0.1 mM, 44% inhibition
Sus scrofa
2.7.1.113
dGTP
0.1 mM, complete inhibition
Sus scrofa
2.7.1.113
dTMP
weak inhibition
Sus scrofa
2.7.1.113
dTTP
0.1 mM, 30% inhibition
Sus scrofa
2.7.1.113
GMP
weak inhibition
Sus scrofa
2.7.1.113
GTP
0.1 mM, 33% inhibition
Sus scrofa
2.7.1.113
additional information
not inhibited by dAMP
Sus scrofa
2.7.1.113
UMP
0.1 mM, 24% inhibition
Sus scrofa
2.7.1.113
UTP
0.1 mM, 30% inhibition
Sus scrofa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.113
0.021
-
dGTP
pH 8.0, 37°C
Sus scrofa
2.7.1.113
0.076
-
dGMP
pH 8.0, 37°C
Sus scrofa
2.7.1.113
0.34
-
deoxyadenosine
pH 8.0, 37°C
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.113
0.00032
-
deoxyguanosine
pH 8.0, 37°C
Sus scrofa
2.7.1.113
3.3
-
MgATP2-
pH 8.0, 37°C
Sus scrofa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.113
Ca2+
67% of activity with Mg2+; activation
Sus scrofa
2.7.1.113
Fe2+
slight activation
Sus scrofa
2.7.1.113
Mg2+
required for activity
Sus scrofa
2.7.1.113
Mn2+
92% of activity with Mg2+
Sus scrofa
2.7.1.113
additional information
divalent cations required, not activated by Zn2+
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.113
58500
-
gel filtration
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.113
pH 5.5, ammonium sulfate, DEAE-Sephadex
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.113
skin
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.113
0.0000338
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.113
ATP + deoxyguanosine
highly specific for phospho group acceptor
640552
Sus scrofa
ADP + dGMP
-
640552
Sus scrofa
?
2.7.1.113
ATP + deoxyguanosine
ATP twice as effective as CTP or GTP and 4 times as effective as UTP
640552
Sus scrofa
ADP + dGMP
-
640552
Sus scrofa
?
2.7.1.113
CTP + deoxyguanosine
50% of activity with ATP
640552
Sus scrofa
CDP + dGMP
-
-
-
?
2.7.1.113
GTP + deoxyguanosine
50% of activity with ATP
640552
Sus scrofa
GDP + dGMP
-
640552
Sus scrofa
?
2.7.1.113
UTP + deoxyguanosine
25% of activity with ATP
640552
Sus scrofa
UDP + dGMP
-
640552
Sus scrofa
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.113
37
-
-
Sus scrofa
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.1.113
30
53
17% loss of activity at 53°C, 50% loss of activity at 30°C and 53°C
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.113
8.5
-
-
Sus scrofa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.113
5
9
approx. 55% of maximal activity at pH 5.5 and pH 9, plateau with approx. 85% of maximal activity at pH 6.0-7.5
Sus scrofa