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Literature summary extracted from

  • Uyeda, K.; Kurooka, S.
    Phosphofructokinase from Clostridium pasteurianum (1975), Methods Enzymol., 42C, 86-91.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 ADP activation Clostridium pasteurianum
2.7.1.11 ADP positive effector Clostridium pasteurianum
2.7.1.11 NH4+ required for activity Clostridium pasteurianum
2.7.1.11 NH4+ can replace K+ Clostridium pasteurianum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.11
-
 Clostridium pasteurianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 additional information not inhibited by ATP Clostridium pasteurianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.055
-
 ATP pH 8.0, 28°C Clostridium pasteurianum
2.7.1.11 0.18
-
 NH4+ pH 8.0, 28°C Clostridium pasteurianum
2.7.1.11 20
-
 K+ pH 8.0, 28°C Clostridium pasteurianum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 K+ required for activity, inhibition at high concentrations is reversed by increased concentrations of ATP Clostridium pasteurianum
2.7.1.11 K+ can replace NH4+ Clostridium pasteurianum
2.7.1.11 Mg2+ required for activity Clostridium pasteurianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 35000
-
 4 * 35000, high speed sedimentation equilibrium centrifugation or PAGE in the presence of 8 M urea or 7 M guanidine Clostridium pasteurianum
2.7.1.11 144000
-
 high speed sedimentation equilibrium analysis Clostridium pasteurianum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Clostridium pasteurianum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 heat treatment, isopropanol, ammonium sulfate, DEAE-cellulose, crystallization Clostridium pasteurianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 160
-
-
 Clostridium pasteurianum

Storage Stability

EC Number Storage Stability Organism
2.7.1.11 2°C, crystalline, in 45% saturated ammonium sulfate, at least 2 months Clostridium pasteurianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Clostridium pasteurianum ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 CTP + D-fructose 6-phosphate
-
 Clostridium pasteurianum CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate
-
 Clostridium pasteurianum GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ITP + D-fructose 6-phosphate
-
 Clostridium pasteurianum IDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 UTP + D-fructose 6-phosphate
-
 Clostridium pasteurianum UDP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 tetramer 4 * 35000, high speed sedimentation equilibrium centrifugation or PAGE in the presence of 8 M urea or 7 M guanidine Clostridium pasteurianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 7 8.2
-
 Clostridium pasteurianum