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Literature summary extracted from

  • Hausler, R.E.; Holtum, J.A.M.; Latzko, E.
    Cytosolic phosphofructokinase from spinach leaves: 2. The roles of magnesium and ATP (1989), Plant Physiol., 90, 1506-1512.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 ADP activation Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ATP free form Spinacia oleracea
2.7.1.11 Ca2+ depending upon total Mg2+-concentration, kinetics, cytosolic isozyme Spinacia oleracea
2.7.1.11 Mg2+ free form, citrate reverses, independent of MgATP-concentration, kinetics, cytosolic isozyme Spinacia oleracea
2.7.1.11 additional information not inhibited by cAMP; not inhibited by UDP, UDPglucose Spinacia oleracea
2.7.1.11 nucleoside triphosphate free form, pH-dependent Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.11 cytosol
-
 Spinacia oleracea 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Mg2+ required for activity Spinacia oleracea
2.7.1.11 Mg2+ MgNTP2- is the active substrate Spinacia oleracea
2.7.1.11 Mg2+ MgATP is the active substrate Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Spinacia oleracea
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Spinacia oleracea ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 CTP + D-fructose 6-phosphate
-
 Spinacia oleracea CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate
-
 Spinacia oleracea GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ITP + D-fructose 6-phosphate
-
 Spinacia oleracea IDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 UTP + D-fructose 6-phosphate
-
 Spinacia oleracea UDP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 25
-
 assay at Spinacia oleracea