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Literature summary extracted from

  • Sumbilla, C.; Waechter, C.J.
    Properties of brain dolichol kinase activity solubilized with a zwitterionic detergent (1985), Arch. Biochem. Biophys., 238, 75-82.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.108 (R)-dolichol (C95) markedly stimulated by addition, Km 0.009 mM Bos taurus
2.7.1.108 (S)-dolichol (C95) markedly stimulated by addition, Km 0.009 mM Bos taurus
2.7.1.108 phosphatidylcholine stimulated by after dialysis Bos taurus
2.7.1.108 phosphatidylethanolamine stimulated by after dialysis Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.108 CDP
-
 Bos taurus
2.7.1.108 dCDP
-
 Bos taurus
2.7.1.108 EDTA
-
 Bos taurus
2.7.1.108 additional information not inhibited by CMP, ADP, GDP or UDP Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.108 0.0077
-
 CTP pH 7.2, 37°C Bos taurus
2.7.1.108 0.0091
-
 dCTP pH 7.2, 37°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.108 microsome
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.108 Ca2+
-
 Bos taurus
2.7.1.108 Zn2+ markedly stimulated by Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.108 CTP + dolichol Bos taurus
-
 CDP + dolichyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.108 Bos taurus
-
 calf
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.108 brain
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.108 -17°C, 65% of original activity retains when stored for 6 weeks Bos taurus
2.7.1.108 4°C, no detectable loss of activity when solubilized extract is kept for 24 h Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.108 CTP + dolichol
-
 Bos taurus CDP + dolichyl phosphate
-
 ?
2.7.1.108 dCTP + dolichol
-
 Bos taurus dCDP + dolichyl phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.108 7.5
-
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.108 0.121
-
 CDP pH 7.2, 37°C Bos taurus