BRENDA - Enzyme Database

Diglyceride kinase from Escherichia coli. Purification in organic solvent and some properties of the enzyme

Bohnenberger, E.; Sandermann, H.; Eur. J. Biochem. 94, 401-407 (1979)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
cardiolipin
activates
Escherichia coli
2.7.1.107
Lipid
purified enzyme is completely inactive unless a lipid is added to the assay buffer containing Triton X-100
Escherichia coli
2.7.1.107
phosphatidylethanolamine
plus cardiolipin, activates
Escherichia coli
2.7.1.107
Span-20
activates
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.107
0.23
-
ceramide
pH 6.6, 25°C
Escherichia coli
2.7.1.107
1.4
-
ATP
pH 6.6, 25°C
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.107
membrane
-
Escherichia coli
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.107
15000
-
1 * 15000, SDS-PAGE
Escherichia coli
2.7.1.107
15400
-
gel filtration
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.107
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.107
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
ATP + ceramide
-
640362
Escherichia coli
ADP + ceramide 3-phosphate
-
-
-
?
2.7.1.107
additional information
no activity with ficaprenol
640362
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.107
monomer
1 * 15000, SDS-PAGE
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.107
6.3
8.3
-
Escherichia coli
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.1.107
Escherichia coli
isoelectric focusing
-
4
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
cardiolipin
activates
Escherichia coli
2.7.1.107
Lipid
purified enzyme is completely inactive unless a lipid is added to the assay buffer containing Triton X-100
Escherichia coli
2.7.1.107
phosphatidylethanolamine
plus cardiolipin, activates
Escherichia coli
2.7.1.107
Span-20
activates
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.107
0.23
-
ceramide
pH 6.6, 25°C
Escherichia coli
2.7.1.107
1.4
-
ATP
pH 6.6, 25°C
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.107
membrane
-
Escherichia coli
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.107
15000
-
1 * 15000, SDS-PAGE
Escherichia coli
2.7.1.107
15400
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.107
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
ATP + ceramide
-
640362
Escherichia coli
ADP + ceramide 3-phosphate
-
-
-
?
2.7.1.107
additional information
no activity with ficaprenol
640362
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.107
monomer
1 * 15000, SDS-PAGE
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.107
6.3
8.3
-
Escherichia coli
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.1.107
Escherichia coli
isoelectric focusing
-
4