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Literature summary extracted from

  • Kanoh, H.; Sakane, F.; Yamada, K.
    Diacylglycerol kinase isozymes from brain and lymphoid tissues (1992), Methods Enzymol., 209, 162-172.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 phosphatidylserine activates Sus scrofa
2.7.1.107 sphingosine potently activates the 80000 Da enzyme Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 R59022 inhibits the 80000 Da enzyme form but not the 150000 Da enzyme form Sus scrofa
2.7.1.107 sphingosine inhibits the 150000 Da enzyme Sus scrofa
2.7.1.107 Triton X-100 extremely inhibitory to the 80000 Da enzyme and the 150000 Da enzyme form Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 cytosol
-
 Sus scrofa 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Ca2+ 2 mol of Ca2+ per mol of enzyme. Ca2+ plus phosphatidylserine markedly activates by reducing the Km value for ATP Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol Sus scrofa the enzyme may regulate the intracellular concentration of diacylglycerol ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol Sus scrofa the 80000 Da and the 150000 Da enzyme form do not possess specificity towards diacylglycerol molecular species ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.107 phosphoprotein the 80000 Da enzyme form is phosphorylated by an unidentified protein kinase Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 a heat-labile 80000 Da enzyme and a heat-stable 150000 Da enzyme Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 brain
-
 Sus scrofa
-
2.7.1.107 lymphoid tissue
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 7.7
-
 1500000 Da enzyme Sus scrofa
2.7.1.107 15
-
 80000 Da enzyme Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.1.107 the 150000 Da enzyme is very unstable but can be stored at -80°C in presence of bovine serum albumin Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol the enzyme may regulate the intracellular concentration of diacylglycerol Sus scrofa ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol the 80000 Da and the 150000 Da enzyme form do not possess specificity towards diacylglycerol molecular species Sus scrofa ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + sn-1,2-dioleoylglycerol
-
 Sus scrofa ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
 ?