EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.101 | CuSO4 | 1 mM, 50% inhibition | Mycobacterium butyricum | |
2.7.1.101 | HgCl2 | 2 mM, 32% inhibition | Mycobacterium butyricum | |
2.7.1.101 | MgCl2 | above 3 mM | Mycobacterium butyricum | |
2.7.1.101 | additional information | not inhibited by 1 mM N-ethylmaleimide, 1 mM iodoacetate, 1 mM NaF, 1 mM EDTA or 1 mM 1,10-phenanthroline | Mycobacterium butyricum | |
2.7.1.101 | p-chloromercuribenzoate | 2 mM, 15% inhibition | Mycobacterium butyricum | |
2.7.1.101 | p-chloromercuriphenylsulfate | 2 mM, 21% inhibition | Mycobacterium butyricum | |
2.7.1.101 | ZnSO4 | 1 mM, 38% inhibition | Mycobacterium butyricum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.101 | 0.8 | - |
D-tagatose | at 22°C, pH 7.5 | Mycobacterium butyricum | |
2.7.1.101 | 1 | - |
ATP | at 22°C, pH 7.5 | Mycobacterium butyricum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.101 | Fe2+ | 2.5 mM FeSO4, activates, can replace Mg2+ with 24% efficiency | Mycobacterium butyricum | |
2.7.1.101 | Mg2+ | requirement, maximum activity in the presence of 3 mM MgCl2, inhibitory above 3 mM | Mycobacterium butyricum | |
2.7.1.101 | Mn2+ | 2.5 mM MnCl2, activates, can replace Mg2+ with 63% efficiency | Mycobacterium butyricum | |
2.7.1.101 | additional information | not activated by CaCl2, CoSO4, NiSO4, CdSO4 or ZnSO4 | Mycobacterium butyricum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.101 | 63000 | - |
gel filtration | Mycobacterium butyricum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.101 | ATP + D-tagatose | Mycobacterium butyricum | part of the dulcitol degradation pathway in saprophytic mycobacteria | ADP + D-tagatose 6-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.101 | Mycobacterium butyricum | - |
dulcitol-grown | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.101 | 106fold, partial | Mycobacterium butyricum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.101 | 3.92 | - |
at 37°C, pH 7.5 | Mycobacterium butyricum |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.101 | room temperature, phosphate buffer, pH 6.3-8, 1 day, stable | Mycobacterium butyricum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.101 | ATP + D-tagatose | highly specific for D-tagatose, phosphorylates D-tagatose at C-6 | Mycobacterium butyricum | ADP + D-tagatose 6-phosphate | - |
ir | |
2.7.1.101 | ATP + D-tagatose | part of the dulcitol degradation pathway in saprophytic mycobacteria | Mycobacterium butyricum | ADP + D-tagatose 6-phosphate | - |
? | |
2.7.1.101 | additional information | no substrates: D-glucose, D-mannose, D-galactose, D-fructose, D-galactosamine, D-2-deoxygalactose, dulcitol, L-sorbose, L-rhamnose, D-ribose, D-xylose, D-lyxose, D-xylulose or glycerol | Mycobacterium butyricum | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.101 | 22 | - |
assay at, spectrophotometric method, measurement of ADP formation | Mycobacterium butyricum |
2.7.1.101 | 37 | - |
assay at, measurement of the rate of disappearance of tagatose | Mycobacterium butyricum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.101 | 48 | - |
1 min, stable, rapid inactivation above | Mycobacterium butyricum |
2.7.1.101 | 50 | - |
rapidly loses activity on heating above | Mycobacterium butyricum |
2.7.1.101 | 56 | - |
t1/2: 1 min | Mycobacterium butyricum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.101 | 7.5 | - |
22°C | Mycobacterium butyricum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.101 | 6.4 | 8.4 | about half-maximal activity at pH 6.4 and 8.4 | Mycobacterium butyricum |