Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Caceres, A.J.; Portillo, R.; Acosta, H.; Rosales, D.; Quinones, W.; Avilan, L.; Salazar, L.; Dubourdieu, M.; Michels, P.A.M.; Concepcion, J.L.
    Molecular and biochemical characterization of hexokinase from Trypanosoma cruzi (2003), Mol. Biochem. Parasitol., 126, 251-262.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.1 pharmacology enzyme may constitute an interesting drug target Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 hexokinase gene is cloned, sequenced and expressed in Escherichia coli XL-1 Blue, gene encodes a polypeptide of 471 amino acids Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 ADP natural hexokinase from epimastigotes Trypanosoma cruzi
2.7.1.1 diphosphate natural hexokinase from epimastigotes, mixed-type inhibition Trypanosoma cruzi
2.7.1.1 additional information not inhibited by D-fructose; not inhibited by D-glucose 6-phosphate; not inhibited by trehalose 6-phosphate Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.043
-
 D-glucose natural hexokinase from epimastigotes, at pH 7.5 Trypanosoma cruzi
2.7.1.1 0.06
-
 D-glucose recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at pH 7.5 Trypanosoma cruzi
2.7.1.1 0.32 0.38 ATP natural and recombinant hexokinase, expressed in Escherichia coli XL-1 Blue, at pH 7.5 Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.1 glycosome in glycosome-enriched fraction Trypanosoma cruzi 20015
-
2.7.1.1 additional information sequence contains an N-terminal peroxisome-targeting signal, PTS-2, subcellular distribution Trypanosoma cruzi
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Mg2+ MgCl2 is most effective, followed by MnCl2 with 16% of the activity found with MgCl2 Trypanosoma cruzi
2.7.1.1 Mg2+ strict requirement for divalent metal ions Trypanosoma cruzi
2.7.1.1 Mn2+ MgCl2 is most effective, followed by MnCl2 with 16% of the activity found with MgCl2 Trypanosoma cruzi
2.7.1.1 Mn2+ strict requirement for divalent metal ions Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 49000
-
 2 * 49000, at high ionic strength the enzyme exists as dimer, SDS-PAGE Trypanosoma cruzi
2.7.1.1 49000
-
 4 * 52000, predominant form, enzyme exists in several aggregation states, calculated from the amino acid sequence, 4 * 49000, SDS-PAGE Trypanosoma cruzi
2.7.1.1 52000
-
 4 * 52000, predominant form, enzyme exists in several aggregation states, calculated from the amino acid sequence, 4 * 49000, SDS-PAGE Trypanosoma cruzi
2.7.1.1 100000
-
 natural hexokinase, dimer, at high ionic strength, enzyme exists in several aggregation states Trypanosoma cruzi
2.7.1.1 180000
-
 recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at low ionic strength, gel filtration Trypanosoma cruzi
2.7.1.1 200000
-
 gel filtration Trypanosoma cruzi
2.7.1.1 200000
-
 natural hexokinase, tetramer, at low ionic strength, enzyme exists in several aggregation states Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 D-glucose + ATP Trypanosoma cruzi glycolytic enzyme D-glucose 6-phosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Trypanosoma cruzi Q8ST54 strains Y and EP, similar to hexokinase IV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 48.6fold: natural hexokinase from epimastigotes, 66.3fold: recombinant hexokinase expressed in Escherichia coli XL-1 Blue Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 epimastigote
-
 Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 9.27
-
 recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at 25°C, pH 7.5 Trypanosoma cruzi
2.7.1.1 19.44
-
 natural hexokinase from epimastigotes, at 25°C, pH 7.5 Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 D-glucose + ATP specific for glucose Trypanosoma cruzi ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP glycolytic enzyme Trypanosoma cruzi D-glucose 6-phosphate + ADP
-
 ?
2.7.1.1 additional information not: diphosphate Trypanosoma cruzi ?
-
 ?
2.7.1.1 additional information not: mannose Trypanosoma cruzi ?
-
 ?
2.7.1.1 additional information not: fructose Trypanosoma cruzi ?
-
 ?
2.7.1.1 additional information not: D-galactose Trypanosoma cruzi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 dimer 2 * 49000, at high ionic strength the enzyme exists as dimer, SDS-PAGE Trypanosoma cruzi
2.7.1.1 tetramer 4 * 52000, predominant form, enzyme exists in several aggregation states, calculated from the amino acid sequence, 4 * 49000, SDS-PAGE Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.1 additional information
-
 additional information natural hexokinase: 68160/min, recombinant hexokinase expressed in Escherichia coli XL-1 Blue: 18913/min, at pH 7.5 Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 7.5
-
 assay at Trypanosoma cruzi
2.7.1.1 7.5 8.8
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.5
-
 diphosphate natural hexokinase from epimastigotes, at pH 7.5 Trypanosoma cruzi
2.7.1.1 0.7
-
 diphosphate recombinant hexokinase expressed in Escherichia coli, at pH 7.5 Trypanosoma cruzi
2.7.1.1 1.3
-
 ADP natural hexokinase from epimastigotes, at pH 7.5 Trypanosoma cruzi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.1 Trypanosoma cruzi calculated basic isoelectric point
-
 9.67