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Literature summary extracted from

  • White, T.K.; Wilson, J.E.
    Isolation and characterization of the discrete N- and C-terminal halves of rat brain hexokinase: retention of full catalytic activity in the isolated C-terminal half (1989), Arch. Biochem. Biophys., 274, 375-393.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.1 storage of purified enzyme in D-glucose-containing phosphate buffer enhances its stability Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 1,5-Anhydro-D-glucitol 6-phosphate intact enzyme and catalytically active 51 kDa C fragment of hexokinase Rattus norvegicus
2.7.1.1 arsenate catalytically active 51 kDa C fragment of hexokinase Rattus norvegicus
2.7.1.1 D-glucose 6-phosphate
-
 Rattus norvegicus
2.7.1.1 phosphate competitive versus ATP; intact enzyme: at high concentrations, catalytically active 51 kDa C fragment of hexokinase: also at low concentrations; noncompetitive to glucose Rattus norvegicus
2.7.1.1 SO42- catalytically active 51 kDa C fragment of hexokinase Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 additional information
-
 retention of the full catalytic activity of rat brain hexokinase C-terminal half supports the suggestion that the 100000 MW hexokinase evolves from an ancestral 50000 MW yeast type hexokinase by a process of gene duplication Rattus norvegicus
2.7.1.1 100000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 D-glucose + ATP Rattus norvegicus initial catalytic step of the cerebral glucose metabolism, glucose 6-phosphate represents a major regulatory influence on the in vivo activity D-glucose 6-phosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Rattus norvegicus
-
 hexokinase type I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 intact hexokinase I, isolation of a catalytically active 51 kDa C fragment and a 52 kDa N fragment without catalytic activity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 brain hexokinase I Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 60
-
 intact 100 kDa enzyme Rattus norvegicus
2.7.1.1 110
-
 catalytically active 51 kDa C fragment of hexokinase Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 D-fructose + ATP poor substrate Rattus norvegicus ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP good substrate Rattus norvegicus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP initial catalytic step of the cerebral glucose metabolism, glucose 6-phosphate represents a major regulatory influence on the in vivo activity Rattus norvegicus D-glucose 6-phosphate + ADP
-
 ?
2.7.1.1 D-mannose + ATP good substrate Rattus norvegicus ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 additional information isolated catalytically active 51 kDa C-fragment and 52 kDa N-fragment without catalytic activity, both fragments contain discrete binding sites for hexoses and hexose 6-phosphates, one of each pair of sites must be latent in the intact enzyme, the regulatory site binding glucose 6-phosphate is associated with the N-terminal half Rattus norvegicus ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.01 0.02 1,5-Anhydro-D-glucitol 6-phosphate catalytically active 51 kDa C fragment of hexokinase Rattus norvegicus
2.7.1.1 6
-
 phosphate catalytically active 51 kDa C fragment of hexokinase, versus ATP Rattus norvegicus
2.7.1.1 35
-
 phosphate intact hexokinase I Rattus norvegicus
2.7.1.1 60
-
 phosphate catalytically active 51 kDa C fragment of hexokinase, versus glucose Rattus norvegicus