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Literature summary extracted from

  • Cooper, A.J.L.
    Purification of soluble and mitochondrial glutamine transaminase K from rat kidney. Use of a sensitive assay involving transamination between L-phenylalanine and alpha-keto-gamma-methiolbutyrate (1978), Anal. Biochem., 89, 451-460.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.64 cytoplasm
-
 Rattus norvegicus 5737
-
2.6.1.64 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.64 48000
-
 2 * 48000, SDS-PAGE Rattus norvegicus
2.6.1.64 100000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.64 L-glutamine + phenylpyruvate Rattus norvegicus
-
 2-oxoglutaramate + L-phenylalanine
-
 r
2.6.1.64 L-glutamine + phenylpyruvate Rattus norvegicus Wistar
-
 2-oxoglutaramate + L-phenylalanine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.64 Rattus norvegicus
-
 Wistar
-
2.6.1.64 Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.64
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.64 brain
-
 Rattus norvegicus
-
2.6.1.64 heart
-
 Rattus norvegicus
-
2.6.1.64 kidney K-form Rattus norvegicus
-
2.6.1.64 liver L-form Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.64 2.65
-
 mitochondrial isozyme Rattus norvegicus
2.6.1.64 6.48
-
 cytoplasmic isozyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.64 4°C, both isozymes stored in 200 mM potassium phosphate buffer, pH 7.2, 20% glycerol and 5 mM 2-mercaptoethanol, stable for at least 2 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.64 L-glutamine + phenylpyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-phenylalanine
-
 r
2.6.1.64 L-glutamine + phenylpyruvate
-
 Rattus norvegicus Wistar 2-oxoglutaramate + L-phenylalanine
-
 r
2.6.1.64 L-phenylalanine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Rattus norvegicus phenylpyruvate + L-methionine
-
 r
2.6.1.64 L-phenylalanine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Rattus norvegicus Wistar phenylpyruvate + L-methionine
-
 r
2.6.1.64 additional information identical with histidine-pyruvate transaminase II and phenylalanine-alpha-keto-gamma-methiolbutyrate transaminase Rattus norvegicus ?
-
 ?
2.6.1.64 additional information identical with histidine-pyruvate transaminase II and phenylalanine-alpha-keto-gamma-methiolbutyrate transaminase Rattus norvegicus Wistar ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.64 dimer 2 * 48000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.64 glutamine transaminase K
-
 Rattus norvegicus
2.6.1.64 glutamine transaminase L
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.64 pyridoxal 5'-phosphate
-
 Rattus norvegicus