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Literature summary extracted from

  • Pathre, U.; Singh, A.K.; Viswanathan, P.N.; Sane, P.V.
    Purification and properties of leucine aminotransferase from soybean seedlings (1987), Phytochemistry, 26, 2913-2917.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.6 adipic acid
-
Glycine max
2.6.1.6 Fumaric acid
-
Glycine max
2.6.1.6 Glutaric acid
-
Glycine max
2.6.1.6 hydroxylamine inhibits the aldehyde form of the enzyme while the amino form is found to be inert Glycine max
2.6.1.6 Maleate
-
Glycine max
2.6.1.6 Maleic acid
-
Glycine max
2.6.1.6 phenylhydrazine
-
Glycine max
2.6.1.6 potassium cyanide
-
Glycine max
2.6.1.6 Sodium bisulfite
-
Glycine max
2.6.1.6 succinic acid
-
Glycine max
2.6.1.6 Thiosemicarbazide
-
Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.6 1.67
-
L-leucine isozyme LAT II, pH 9, 37°C Glycine max
2.6.1.6 2.3
-
2-oxoglutarate isozyme LAT II, pH 9, 37°C Glycine max
2.6.1.6 2.5
-
L-leucine isozyme LAT I, pH 9, 37°C Glycine max
2.6.1.6 3.3
-
2-oxoglutarate isozyme LAT I, pH 9, 37°C Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.6 68000
-
gel filtration, isoenzyme LAT I Glycine max
2.6.1.6 93300
-
gel filtration, isoenzyme LAT II Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.6 L-leucine + 2-oxoglutarate Glycine max
-
4-methyl-2-oxopentanoate + L-glutamate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.6 Glycine max
-
soybean, var. Kali tur
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.6 partially, 2 isoenzymes, LAT I and LAT II Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.6 seedling
-
Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.6 7.4
-
isoenzyme LAT I Glycine max
2.6.1.6 8.7
-
isoenzyme LAT II Glycine max

Storage Stability

EC Number Storage Stability Organism
2.6.1.6 4°C, concentrated enzyme, activity is stable for 2 months Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.6 L-leucine + 2-oxoglutarate
-
Glycine max 4-methyl-2-oxopentanoate + L-glutamate
-
?
2.6.1.6 L-leucine + 2-oxoglutarate
-
Glycine max 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.6 L-leucine + 4-methyl-2-oxopentanoate
-
Glycine max 4-methyl-2-oxopentanoate + L-leucine
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.6 8.9
-
-
Glycine max