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Literature summary extracted from

  • Dietrich, J.B.; Lorber, B.; Kern, D.
    Expression of mammalian tyrosine aminotransferase in Saccharomyces cerevisiae and Escherichia coli. Purification to homogeneity and characterization of the enzyme overproduced in the bacteria (1991), Eur. J. Biochem., 201, 399-407.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.5 expression of rat liver enzyme in Saccharomyces cerevisiae and Escherichia coli Rattus norvegicus

General Stability

EC Number General Stability Organism
2.6.1.5 pyridoxal 5'-phosphate protects against thermal inactivation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.5 Canaline
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 0.7
-
 2-oxoglutarate pH 7.2, 37°C Rattus norvegicus
2.6.1.5 3.4
-
 L-tyrosine pH 7.2, 37°C Rattus norvegicus
2.6.1.5 60
-
 tryptophan pH 7.2, 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.5 50000
-
 2 * 50000, SDS-PAGE Rattus norvegicus
2.6.1.5 110000
-
 recombinant expressed in Escherichia coli, ultracentrifugation Rattus norvegicus
2.6.1.5 150000
-
 recombinant expressed in Escherichia coli, native gradient PAGE Rattus norvegicus
2.6.1.5 160000
-
 recombinant expressed in Escherichia coli, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.5 partial purification of enzyme expressed in Saccharomyces cerevisiae and Escherichia coli using affinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.5 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.5 additional information
-
-
 Rattus norvegicus
2.6.1.5 416
-
 enzyme expressed in Escherichia coli Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 L-phenylalanine + 2-oxoglutarate
-
 Rattus norvegicus phenylpyruvate + L-glutamate
-
 ?
2.6.1.5 L-tyrosine + 2-oxoglutarate
-
 Rattus norvegicus 4-hydroxyphenylpyruvate + L-glutamate
-
 ?
2.6.1.5 tryptophan + 2-oxoglutarate
-
 Rattus norvegicus 3-indole-2-oxopropanoate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.5 dimer 2 * 50000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.5 additional information
-
 pyridoxal 5'-phosphate protects against thermal inactivation Rattus norvegicus
2.6.1.5 additional information
-
 the apoenzyme is thermally unstable: t1/2 of 2 min at 55°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.5 7.7
-
 recombinant enzyme expressed in Escherichia coli Rattus norvegicus