BRENDA - Enzyme Database show

The biosynthesis of ectoine

Peters, P.; Galinski, E.A.; Trper, H.G.; FEMS Microbiol. Lett. 71, 157-162 (1990)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.46
1.2
-
L-glutamate
pH 9.1, 37C
Halorhodospira halochloris
2.6.1.46
1.85
-
L-glutamate
-
Halomonas elongata
2.6.1.46
1.85
-
L-glutamate
pH 9.1, 37C
Halorhodospira halochloris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
Halorhodospira halochloris
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halorhodospira halochloris
?
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
Halomonas elongata
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halomonas elongata
?
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
Halorhodospira halochloris
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
r
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
Halomonas elongata
final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M
L-ectoine + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.178
Halorhodospira halochloris
-
-
-
2.6.1.46
Halomonas elongata
-
strain DSM 2581
-
2.6.1.46
Halorhodospira halochloris
-
strain DSM 1059
-
4.2.1.108
Halomonas elongata
-
extremely halophilic eubacterium, strain DSM 2581
-
4.2.1.108
Halorhodospira halochloris
-
extremely halophilic eubacterium, strain DSM 1059
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.108
cell culture
photosynthetically grown cells
Halorhodospira halochloris
-
4.2.1.108
culture condition:glucose-grown cell
-
Halomonas elongata
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.178
0.114
-
-
Halorhodospira halochloris
2.6.1.46
0.054
-
-
Halorhodospira halochloris
2.6.1.46
0.171
-
-
Halomonas elongata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.178
acetyl-CoA + L-2,4-diaminobutanoate
-
640090
Halorhodospira halochloris
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
-
640090
Halorhodospira halochloris
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halorhodospira halochloris
?
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
-
640090
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halomonas elongata
?
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
640090
Halorhodospira halochloris
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halorhodospira halochloris
?
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
640090
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halomonas elongata
?
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
640090
Halorhodospira halochloris
L-ectoine + H2O
-
-
-
r
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M
640090
Halomonas elongata
L-ectoine + H2O
-
-
-
r
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
640090
Halomonas elongata
L-ectoine + H2O
NMR and thin layer chromatography product identification
-
-
r
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
640090
Halorhodospira halochloris
L-ectoine + H2O
NMR and TLC product identification
-
-
r
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.178
35
40
-
Halorhodospira halochloris
2.6.1.46
37
-
assay at
Halomonas elongata
2.6.1.46
37
-
assay at
Halorhodospira halochloris
4.2.1.108
37
-
assay at
Halomonas elongata
4.2.1.108
37
-
assay at
Halorhodospira halochloris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.178
8.2
-
-
Halorhodospira halochloris
2.6.1.46
7.9
-
-
Halomonas elongata
2.6.1.46
8.2
-
-
Halorhodospira halochloris
4.2.1.108
9
-
assay at
Halomonas elongata
4.2.1.108
9
-
assay at
Halorhodospira halochloris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.46
1.2
-
L-glutamate
pH 9.1, 37C
Halorhodospira halochloris
2.6.1.46
1.85
-
L-glutamate
-
Halomonas elongata
2.6.1.46
1.85
-
L-glutamate
pH 9.1, 37C
Halorhodospira halochloris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
Halorhodospira halochloris
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halorhodospira halochloris
?
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
Halomonas elongata
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halomonas elongata
?
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
Halorhodospira halochloris
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
r
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
Halomonas elongata
final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M
L-ectoine + H2O
-
-
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.108
cell culture
photosynthetically grown cells
Halorhodospira halochloris
-
4.2.1.108
culture condition:glucose-grown cell
-
Halomonas elongata
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.178
0.114
-
-
Halorhodospira halochloris
2.6.1.46
0.054
-
-
Halorhodospira halochloris
2.6.1.46
0.171
-
-
Halomonas elongata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.178
acetyl-CoA + L-2,4-diaminobutanoate
-
640090
Halorhodospira halochloris
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
-
640090
Halorhodospira halochloris
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halorhodospira halochloris
?
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
-
640090
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halomonas elongata
?
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
640090
Halorhodospira halochloris
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halorhodospira halochloris
?
2.6.1.46
L-2-amino-4-oxobutanoate + L-glutamate
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
640090
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halomonas elongata
?
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
640090
Halorhodospira halochloris
L-ectoine + H2O
-
-
-
r
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M
640090
Halomonas elongata
L-ectoine + H2O
-
-
-
r
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
640090
Halomonas elongata
L-ectoine + H2O
NMR and thin layer chromatography product identification
-
-
r
4.2.1.108
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
640090
Halorhodospira halochloris
L-ectoine + H2O
NMR and TLC product identification
-
-
r
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.178
35
40
-
Halorhodospira halochloris
2.6.1.46
37
-
assay at
Halomonas elongata
2.6.1.46
37
-
assay at
Halorhodospira halochloris
4.2.1.108
37
-
assay at
Halomonas elongata
4.2.1.108
37
-
assay at
Halorhodospira halochloris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.178
8.2
-
-
Halorhodospira halochloris
2.6.1.46
7.9
-
-
Halomonas elongata
2.6.1.46
8.2
-
-
Halorhodospira halochloris
4.2.1.108
9
-
assay at
Halomonas elongata
4.2.1.108
9
-
assay at
Halorhodospira halochloris