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Literature summary extracted from

  • Hoare, K.; Datta, K.
    Characteristics of L-alanine:4,5-dioxovaleric acid transaminase: an alternate pathway of heme biosynthesis in yeast (1990), Arch. Biochem. Biophys., 277, 122-129.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.43 repeated freezing and thawing inactivates the enzyme, stability of the enzyme is enhanced by presence of 5% glycerol Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.43 additional information EDTA, N-ethylmaleimide. iodoacetic acid, cysteine and beta-mercaptoethanol are no inhibitors Candida albicans
2.6.1.43 p-chloromercuribenzoate
-
 Candida albicans
2.6.1.43 Tris-HCl
-
 Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.43 0.00028
-
 L-alanine pH 7.2, 60°C Candida albicans
2.6.1.43 0.0105
-
 4,5-Dioxopentanoate pH 7.2, 60°C Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.43 cytosol
-
 Candida albicans 5829
-
2.6.1.43 mitochondrion
-
 Candida albicans 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.43 57544
-
 1 * 57544, SDS-PAGE Candida albicans
2.6.1.43 59000
-
 gel filtration Saccharomyces cerevisiae
2.6.1.43 59000
-
 gel filtration Candida albicans
2.6.1.43 60260
-
 gel filtration Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.43 L-alanine + 4,5-dioxopentanoate Saccharomyces cerevisiae
-
 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate Candida albicans
-
 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate Candida albicans 3100
-
 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate Saccharomyces cerevisiae 3059 S288C
-
 5-aminolevulinate + pyruvate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.43 Candida albicans
-
 3100
-
2.6.1.43 Candida albicans 3100
-
 3100
-
2.6.1.43 Saccharomyces cerevisiae
-
 3059, S288C
-
2.6.1.43 Saccharomyces cerevisiae 3059 S288C
-
 3059, S288C
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.43
-
 Saccharomyces cerevisiae
2.6.1.43
-
 Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.43 0.186
-
-
 Candida albicans

Storage Stability

EC Number Storage Stability Organism
2.6.1.43 4°C, stored in ice for 3 months without any appreciable loss of enzyme activity Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.43 glycine + 4,5-dioxopentanoate
-
 Candida albicans 5-aminolevulinate + glyoxylate
-
 ir
2.6.1.43 glycine + 4,5-dioxopentanoate
-
 Candida albicans 3100 5-aminolevulinate + glyoxylate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Saccharomyces cerevisiae 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Candida albicans 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Candida albicans 3100 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Saccharomyces cerevisiae 3059 S288C 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-arginine + 4,5-dioxopentanoate 6.8% of the activity with L-alanine Candida albicans 5-aminolevulinate + 5-guanidino-2-oxopentanoate
-
 ir
2.6.1.43 L-asparagine + 4,5-dioxopentanoate 74% of the activity with L-alanine Candida albicans 5-aminolevulinate + 4-amino-2,4-dioxobutanoic acid
-
 ir
2.6.1.43 L-aspartate + 4,5-dioxopentanoate 39% of the activity with L-alanine Candida albicans 5-aminolevulinate + oxaloacetate
-
 ir
2.6.1.43 L-glutamate + 4,5-dioxopentanoate 39% of the activity with L-alanine Candida albicans 5-aminolevulinate + 2-oxoglutarate
-
 ir
2.6.1.43 L-glutamate + 4,5-dioxopentanoate 39% of the activity with L-alanine Candida albicans 3100 5-aminolevulinate + 2-oxoglutarate
-
 ir
2.6.1.43 L-glutamine + 4,5-dioxopentanoate 74% of the activity with L-alanine Candida albicans 5-aminolevulinate + 2-oxoglutaramate
-
 ?
2.6.1.43 additional information cannot utilize 2-oxoglutarate nor pyruvate as amino acceptors Candida albicans ?
-
 ?
2.6.1.43 additional information cannot utilize 2-oxoglutarate nor pyruvate as amino acceptors Candida albicans 3100 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.43 monomer 1 * 59000, SDS-PAGE Saccharomyces cerevisiae
2.6.1.43 monomer 1 * 57544, SDS-PAGE Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.43 60
-
-
 Saccharomyces cerevisiae
2.6.1.43 60
-
-
 Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.43 7.2
-
-
 Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.43 pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae
2.6.1.43 pyridoxal 5'-phosphate Km 0.0005 mM Candida albicans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.43 Candida albicans isoelectric focusing using Phast GEl IEF 3-9 4.8 4.7