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Literature summary extracted from

  • Conway, M.E.; Hutson, S.M.
    Mammalian branched-chain aminotransferases (2000), Methods Enzymol., 324, 355-365.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42
-
 Rattus norvegicus
2.6.1.42 vector pET-28a, overexpression of recombinant BCATm and BCATc in Escherichia coli Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.42 cytosol
-
 Homo sapiens 5829
-
2.6.1.42 cytosol
-
 Rattus norvegicus 5829
-
2.6.1.42 mitochondrion
-
 Homo sapiens 5739
-
2.6.1.42 mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Homo sapiens
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate Rattus norvegicus
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Homo sapiens
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-leucine + 2-oxoglutarate Rattus norvegicus
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Rattus norvegicus
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Homo sapiens
-
 human
-
2.6.1.42 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 recombinant Bcatm and BCATc, expressed in E. coli Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 85
-
 recombinant cytosolic enzyme Homo sapiens
2.6.1.42 125
-
 recombinant mitochondrial enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Homo sapiens 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Rattus norvegicus 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Homo sapiens 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Homo sapiens 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Homo sapiens 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Rattus norvegicus 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 BCATc
-
 Homo sapiens
2.6.1.42 BCATc
-
 Rattus norvegicus
2.6.1.42 BCATm
-
 Homo sapiens
2.6.1.42 BCATm
-
 Rattus norvegicus