Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kido, R.
    Pancreatic branched-chain-amino-acid aminotransferase (1988), Methods Enzymol., 166, 275-281.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.42 2-mercaptoethanol protects against inactivation by sulfhydryl reagents Homo sapiens
2.6.1.42 2-mercaptoethanol protects against inactivation by sulfhydryl reagents Rattus norvegicus
2.6.1.42 2-mercaptoethanol protects against inactivation by sulfhydryl reagents Canis lupus familiaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.42 hydrazine
-
 Canis lupus familiaris
2.6.1.42 hydrazine
-
 Homo sapiens
2.6.1.42 hydrazine
-
 Rattus norvegicus
2.6.1.42 hydroxylamine
-
 Canis lupus familiaris
2.6.1.42 hydroxylamine
-
 Homo sapiens
2.6.1.42 hydroxylamine
-
 Rattus norvegicus
2.6.1.42 KCN
-
 Homo sapiens
2.6.1.42 additional information no inhibition with isonicotinic acid hydrazide or KCN Canis lupus familiaris
2.6.1.42 additional information not inhibited by isonicotinic acid and semicarbazide Homo sapiens
2.6.1.42 additional information no inhibition with isonicotinic acid hydrazide or KCN Rattus norvegicus
2.6.1.42 p-chloromercuribenzoate isoenzyme I, complete inhibition, isoenzyme III only partially inhibited Canis lupus familiaris
2.6.1.42 p-chloromercuribenzoate isoenzyme I, complete inhibition, isoenzyme III only partially inhibited Homo sapiens
2.6.1.42 p-chloromercuribenzoate 5 mM, complete inhibition Rattus norvegicus
2.6.1.42 Semicarbazide isoenzyme II is somewhat sensitive, isoenzyme I is not Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 1
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme III, leucine as amino group donor Homo sapiens
2.6.1.42 1
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme III, isoleucine as amino group donor Canis lupus familiaris
2.6.1.42 1.1
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme III, isoleucine as amino group donor Homo sapiens
2.6.1.42 1.4
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme III, leucine as amino group donor Canis lupus familiaris
2.6.1.42 1.7
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme III, valine as amino group donor Homo sapiens
2.6.1.42 1.7
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme III, valine as amino group donor Canis lupus familiaris
2.6.1.42 2.1
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme I, leucine as amino group donor Canis lupus familiaris
2.6.1.42 2.3
-
 L-leucine pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor Canis lupus familiaris
2.6.1.42 2.7
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme I, valine as amino group donor Canis lupus familiaris
2.6.1.42 3
-
 L-valine pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor Canis lupus familiaris
2.6.1.42 3
-
 L-isoleucine pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor Homo sapiens
2.6.1.42 3.6
-
 L-leucine pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor Canis lupus familiaris
2.6.1.42 4
-
 2-oxoglutarate pH 8.0, 37°C, leucine as amino group donor Rattus norvegicus
2.6.1.42 4.2
-
 L-isoleucine pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor Canis lupus familiaris
2.6.1.42 4.8
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme I, isoleucine as amino group donor Homo sapiens
2.6.1.42 5
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme I, valine as amino group donor Homo sapiens
2.6.1.42 6.8
-
 L-leucine pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor Homo sapiens
2.6.1.42 7.4
-
 L-valine pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor Canis lupus familiaris
2.6.1.42 7.6
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme I, leucine as amino group donor Homo sapiens
2.6.1.42 8.3
-
 L-valine pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor Homo sapiens
2.6.1.42 10
-
 2-oxoglutarate pH 8.0, 37°C, isoenzyme I, isoleucine as amino group donor Canis lupus familiaris
2.6.1.42 10.3
-
 L-isoleucine pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor Homo sapiens
2.6.1.42 11.1
-
 L-leucine pH 8.0, 37°C Rattus norvegicus
2.6.1.42 12.5
-
 2-oxoglutarate pH 8.0, 37°C, valine as amino group donor Rattus norvegicus
2.6.1.42 14.2
-
 2-oxoglutarate pH 8.0, 37°C, isoleucine as amino group donor Rattus norvegicus
2.6.1.42 22.2
-
 L-isoleucine pH 8.0, 37°C Rattus norvegicus
2.6.1.42 22.2
-
 L-isoleucine pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor Canis lupus familiaris
2.6.1.42 25
-
 L-leucine pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor Homo sapiens
2.6.1.42 30.8
-
 L-valine pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor Homo sapiens
2.6.1.42 143
-
 L-valine pH 8.0, 37°C, 2-oxoglutarate as amino group acceptor Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 35000
-
 2 * 35000, SDS-PAGE Rattus norvegicus
2.6.1.42 39000
-
 2 * 39000, isoenzyme I, SDS-PAGE Canis lupus familiaris
2.6.1.42 43000
-
 2 * 43000, isoenzyme III, SDS-PAGE Canis lupus familiaris
2.6.1.42 68000
-
 gel filtration Rattus norvegicus
2.6.1.42 79000
-
 sucrose density gradient centrifugation Rattus norvegicus
2.6.1.42 80000
-
 isoenzyme I, gel filtration Homo sapiens
2.6.1.42 80000
-
 isoenzyme I, gel filtration Canis lupus familiaris
2.6.1.42 90000
-
 isoenzyme III, gel filtration Homo sapiens
2.6.1.42 96000
-
 isoenzyme III, gel filtration Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Homo sapiens
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate Rattus norvegicus
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Rattus norvegicus
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Canis lupus familiaris
-
 dog
-
2.6.1.42 Homo sapiens
-
 human
-
2.6.1.42 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42
-
 Homo sapiens
2.6.1.42
-
 Rattus norvegicus
2.6.1.42
-
 Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.42 pancreas
-
 Homo sapiens
-
2.6.1.42 pancreas
-
 Rattus norvegicus
-
2.6.1.42 pancreas
-
 Canis lupus familiaris
-
2.6.1.42 skeletal muscle
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 21.8
-
-
 Rattus norvegicus
2.6.1.42 74
-
-
 Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Homo sapiens 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Rattus norvegicus 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Canis lupus familiaris L-glutamate + 3-methyl-2-oxopentanoate
-
 ?
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Homo sapiens 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Canis lupus familiaris L-glutamate + 4-methyl-2-oxopentanoate
-
 ?
2.6.1.42 L-valine + 2-oxoglutarate
-
 Homo sapiens 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Rattus norvegicus 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Rattus norvegicus 2-oxoisopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Canis lupus familiaris L-glutamate + 3-methyl-2-oxobutanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.42 dimer
-
 Homo sapiens
2.6.1.42 dimer 2 * 35000, SDS-PAGE Rattus norvegicus
2.6.1.42 dimer 2 * 43000, isoenzyme III, SDS-PAGE Canis lupus familiaris
2.6.1.42 dimer 2 * 39000, isoenzyme I, SDS-PAGE Canis lupus familiaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.42 50 65 labile to heat, activity is completely lost by heating at 65°C for 1 min, about 30% is lost within 1 min at 50°C and about 70% within 10 min Rattus norvegicus
2.6.1.42 65
-
 not inactivated by heating for 10 min Homo sapiens
2.6.1.42 65
-
 not inactivated by heating for 10 min Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.42 7.5 8
-
 Rattus norvegicus
2.6.1.42 8
-
 isoenzyme III Homo sapiens
2.6.1.42 8.5 9.5 isoenzyme III Canis lupus familiaris
2.6.1.42 9
-
 isoenzyme I Homo sapiens
2.6.1.42 9
-
 isoenzyme I Canis lupus familiaris