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Literature summary extracted from

  • Lee-Peng, F.C.; Hermodson, M.A.; Kohlhaw, G.B.
    Transaminase B from Escherichia coli: quaternary structure, amino-terminal sequence, substrate specificity, and absence of a separate valine-alpha-ketoglutarate activity (1979), J. Bacteriol., 139, 339-345.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 K-12 strain carries the ilvE gene both on the host chromosome and on a plasmid Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 0.2
-
 DL-2-oxo-3-methyl-n-pentanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 0.52
-
 L-isoleucine pH 8.0, 37°C Escherichia coli
2.6.1.42 0.56
-
 2-Oxoisohexanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 0.56
-
 2-oxoisopentanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 0.58
-
 L-leucine pH 8.0, 37°C Escherichia coli
2.6.1.42 1.28
-
 2-oxoglutarate pH 8.0, 37°C Escherichia coli
2.6.1.42 3.13
-
 L-valine pH 8.0, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 31500
-
 6 * 31500, SDS-PAGE Escherichia coli
2.6.1.42 182300
-
 sedimentation equilibrium centrifugation Escherichia coli
2.6.1.42 185000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Escherichia coli
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Escherichia coli
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Escherichia coli
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 15.9
-
 substrate L-valine + 2-oxoglutarate Escherichia coli
2.6.1.42 27.3
-
 substrate L-isoleucine + 2-oxoglutarate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Escherichia coli 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Escherichia coli 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-phenylalanine + 2-oxoglutarate
-
 Escherichia coli phenylpyruvate + L-glutamate
-
 r
2.6.1.42 L-tyrosine + 2-oxoglutarate
-
 Escherichia coli p-hydroxyphenylpyruvate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Escherichia coli 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 additional information purified enzyme has no measurable L-aspartate-2-oxoglutarate activity Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.42 hexamer 6 * 31500, SDS-PAGE Escherichia coli