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Literature summary extracted from

  • Collins, M.; Wagner, R.P.
    Branded chain amino acid aminotransferases of Neurospora crassa (1973), Arch. Biochem. Biophys., 155, 184-193.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.42 cytosol
-
 Neurospora crassa 5829
-
2.6.1.42 mitochondrion inner membrane-matrix Neurospora crassa 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 67500
-
 mitochondrial isoenzyme, gel filtration Neurospora crassa
2.6.1.42 88000
-
 cytoplasmic isoenzyme, gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Neurospora crassa
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Neurospora crassa mitochondrial isoenzyme functions in the biosynthesis of L-isoleucine, L-leucine and L-valine, cytoplasmic isoenzyme functions in amino acid catabolism 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Neurospora crassa
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 partially Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 25
-
 cytoplasmic enzyme Neurospora crassa
2.6.1.42 48.8
-
 mitochondrial enzyme Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-glutamate + 2-oxoglutarate mitochondrial enzyme Neurospora crassa 2-oxoglutarate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Neurospora crassa 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate mitochondrial enzyme Neurospora crassa 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Neurospora crassa 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate mitochondrial isoenzyme functions in the biosynthesis of L-isoleucine, L-leucine and L-valine, cytoplasmic isoenzyme functions in amino acid catabolism Neurospora crassa 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-methionine + 2-oxoglutarate cytoplasmic isoenzyme Neurospora crassa 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-phenylalanine + 2-oxoglutarate cytoplasmic isoenzyme Neurospora crassa phenylpyruvate + L-glutamate
-
 r
2.6.1.42 L-tyrosine + 2-oxoglutarate cytoplasmic isoenzyme Neurospora crassa p-hydroxyphenylpyruvate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Neurospora crassa 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate mitochondrial enzyme Neurospora crassa 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 additional information purified mitochondrial enzyme is not active with L-phenylalanine Neurospora crassa ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.42 8.5
-
 cytoplasmic enzyme Neurospora crassa
2.6.1.42 8.8
-
 mitochondrial enzyme Neurospora crassa