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Literature summary extracted from

  • Taylor, R.T.; Jenkins, W.T.
    Leucine aminotransferase. II. Purification and characterization (1966), J. Biol. Chem., 241, 4396-4405.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.42 2-mercaptoethanol
-
 Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.42 hydroxylamine
-
 Sus scrofa
2.6.1.42 phenylhydrazine
-
 Sus scrofa
2.6.1.42 potassium cyanide
-
 Sus scrofa
2.6.1.42 Thiosemicarbazide
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 75000
-
 sedimentation equilibrium Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Sus scrofa
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Sus scrofa
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Sus scrofa
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Sus scrofa
-
 pig, hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.42 heart heart muscle Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 50 53
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.6.1.42 -10°C, concentrated solution frozen for 1month retains 90% of her original activity Sus scrofa
2.6.1.42 5°C, pH 7.0, purified enzyme loses activity, about 20% in 1 week Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 DL-2-aminoadipate + 2-oxoglutarate heart enzyme Sus scrofa 3-oxohexanedioic acid + L-glutamate
-
 r
2.6.1.42 DL-2-aminopimelate + 2-oxoglutarate heart enzyme Sus scrofa 2-oxoheptanedioate + L-glutamate
-
 r
2.6.1.42 DL-allo-isoleucine + 2-oxoglutarate heart enzyme Sus scrofa 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-2-aminobutyrate + 2-oxoglutarate heart enzyme Sus scrofa 2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Sus scrofa 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Sus scrofa 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-methionine + 2-oxoglutarate
-
 Sus scrofa 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-norleucine + 2-oxoglutarate
-
 Sus scrofa 2-oxohexanoate + L-glutamate
-
 r
2.6.1.42 L-norvaline + 2-oxoglutarate
-
 Sus scrofa 2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Sus scrofa 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 additional information activity less than 3% with glycine, L-alanine, L-lysine, L-phenylalanine, L-tryptophan, D-alloisoleucine, D-valine, D-leucine, L-threonine, L-histidine, L-arginine, L-cysteine, DL-homocyteine, beta-aminoisobutyrate, gamma-aminobutyrate, alpha-aminoisobutyrate, DL-alpha-aminocaprylate, L-serine, DL-homoserine, DL-beta-aminobutyrate, DL-N-hydroxyleucine, DL-N-hydroxyvaline, L-aspartate, L-tyrosine, L-kynurenine, L-ornithine, DL-proline, L-glutamine, L-peniclliamine, DL-methionine sulfone, DL-methionine sulfoxide and DL-beta-hydroxy-leucine Sus scrofa ?
-
 ?
2.6.1.42 S-methyl-L-cysteine + 2-oxoglutarate heart enzyme Sus scrofa 3-(methylthio)-2-oxopropanoic acid + L-glutamate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 EC 2.6.1.6 related Sus scrofa
2.6.1.42 leucine transaminase
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.42 8.3 8.5
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.42 6 10
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.42 6 7 most stable in this range Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate
-
 Sus scrofa