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Literature summary extracted from

  • Ichihara, A.; Koyama, E.
    Transaminase of branched chain amino acids. I. Branched chain amino acids-alpha-ketoglutarate transaminase (1966), J. Biochem., 59, 160-169.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.42 pyridoxal 5'-phosphate and mercaptoethanol stabilizes the heart enzyme, addition of 2-oxoglutarate does not increase its stability Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.42 isoleucine competitive inhibition of enzyme activity with leucine Sus scrofa
2.6.1.42 p-chloromercuribenzoate
-
 Rattus norvegicus
2.6.1.42 p-chloromercuribenzoate 0.1 mM, complete inhibition Sus scrofa
2.6.1.42 valine competitive inhibition of enzyme activity with leucine Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 0.63
-
 2-oxoglutarate pH 8.6, 25°C, heart enzyme Sus scrofa
2.6.1.42 3.8
-
 L-leucine pH 8.6, 25°C Sus scrofa
2.6.1.42 3.8
-
 L-isoleucine pH 8.6, 25°C Sus scrofa
2.6.1.42 11
-
 L-valine pH 8.6, 25°C Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.42 cytoplasm
-
 Rattus norvegicus 5737
-
2.6.1.42 cytoplasm
-
 Sus scrofa 5737
-
2.6.1.42 microsome
-
 Rattus norvegicus
-
-
2.6.1.42 mitochondrion
-
 Rattus norvegicus 5739
-
2.6.1.42 mitochondrion
-
 Sus scrofa 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Rattus norvegicus
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate Sus scrofa
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Rattus norvegicus
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Sus scrofa
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Rattus norvegicus
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Sus scrofa
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Rattus norvegicus
-
-
-
2.6.1.42 Sus scrofa
-
 pig, hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 hog heart, partial Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.42 heart heart muscle Rattus norvegicus
-
2.6.1.42 heart heart muscle Sus scrofa
-
2.6.1.42 kidney
-
 Rattus norvegicus
-
2.6.1.42 kidney
-
 Sus scrofa
-
2.6.1.42 liver only slight activity Rattus norvegicus
-
2.6.1.42 liver only slight activity Sus scrofa
-
2.6.1.42 skeletal muscle
-
 Rattus norvegicus
-
2.6.1.42 skeletal muscle
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 1.82
-
 heart muscle enzyme, substrate L-leucine Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Rattus norvegicus 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Sus scrofa 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Sus scrofa 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-norleucine + 2-oxoglutarate
-
 Rattus norvegicus 2-oxohexanoate + L-glutamate
-
 r
2.6.1.42 L-norleucine + 2-oxoglutarate
-
 Sus scrofa 2-oxohexanoate + L-glutamate
-
 r
2.6.1.42 L-norvaline + 2-oxoglutarate heart enzyme Sus scrofa 2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Rattus norvegicus 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Sus scrofa 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 additional information L-isoleucine is the best substrate for the heart enzyme, L-alanine, aspartic, alpha-amino-butyric acid and gamma-aminobutyric acid, epsilon-aminocaproic acid, L-ornithine, L-methionine and L-phenylalanine are no substrates, pyruvate is not a good acceptor Sus scrofa ?
-
 ?
2.6.1.42 additional information L-leucine is the best substrate for the heart enzyme Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 branched chain amino acid: 2-oxoglutarate aminotransferase EC 2.6.1 formerly Rattus norvegicus
2.6.1.42 branched chain amino acid: 2-oxoglutarate aminotransferase EC 2.6.1 formerly Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.42 30 60
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.42 30 60 enzyme is inactivated by heat Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.42 8.6
-
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.42 8
-
 hog heart enzyme is rather unstable at pH 8.0 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate
-
 Rattus norvegicus
2.6.1.42 pyridoxal 5'-phosphate Km 0.067 mM Sus scrofa