BRENDA - Enzyme Database

Purification and properties of 2-aminoadipate: 2-oxoglutarate aminotransferase from bovine kidney

Deshmukh, D.R.; Mungre, S.M.; Biochem. J. 261, 761-768 (1989)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.6.1.39
0.1 mM dithiothreitol improves the stability of the enzyme by 50%
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.39
3-methylglutaric acid
6 mM, 2% inhibition
Bos taurus
2.6.1.39
3-methylglutaric acid
6 mM, 38% inhibition
Rattus norvegicus
2.6.1.39
adipic acid
6 mM, 48% inhibition
Bos taurus
2.6.1.39
adipic acid
6 mM, 60% inhibition
Rattus norvegicus
2.6.1.39
azelaic acid
6 mM, 36% inhibition
Bos taurus
2.6.1.39
azelaic acid
6 mM, 38% inhibition
Rattus norvegicus
2.6.1.39
Decanoic acid
6 mM, 50% inhibition
Bos taurus
2.6.1.39
Decanoic acid
6 mM, 45% inhibition
Rattus norvegicus
2.6.1.39
Diethylglutaric acid
6 mM, 20% inhibition
Bos taurus
2.6.1.39
Diethylglutaric acid
6 mM, 20% inhibition
Rattus norvegicus
2.6.1.39
dimethylglutaric acid
6 mM, 15% inhibition
Bos taurus
2.6.1.39
dimethylglutaric acid
6 mM, 42% inhibition
Rattus norvegicus
2.6.1.39
Glutaric acid
6 mM, 5% inhibition
Rattus norvegicus
2.6.1.39
Kynurenic acid
1 mM, 30% inhibition
Bos taurus
2.6.1.39
Kynurenic acid
1 mM, 40% inhibition
Rattus norvegicus
2.6.1.39
additional information
oxaloacetic acid is no inhibitor
Bos taurus
2.6.1.39
additional information
oxaloacetic acid is no inhibitor
Rattus norvegicus
2.6.1.39
pimelic acid
6 mM, 28% inhibition
Bos taurus
2.6.1.39
pimelic acid
6 mM, 32% inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.39
0.5
-
2-oxoadipic acid
pH 7.0, 37°C
Bos taurus
2.6.1.39
0.5
-
2-oxoadipic acid
pH 7.0, 37°C
Rattus norvegicus
2.6.1.39
4.6
-
L-Glutamic acid
pH 7.0, 37°C
Bos taurus
2.6.1.39
4.6
-
L-Glutamic acid
pH 7.0, 37°C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.39
cytoplasm
-
Bos taurus
5737
-
2.6.1.39
cytoplasm
-
Rattus norvegicus
5737
-
2.6.1.39
mitochondrion
-
Bos taurus
5739
-
2.6.1.39
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.39
41000
-
2 * 41000, SDS-PAGE
Bos taurus
2.6.1.39
41000
-
2 * 41000, SDS-PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
Rattus norvegicus
lysine catabolic pathway
2-oxoadipate + L-glutamate
-
-
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
Bos taurus
lysine catabolic pathway
2-oxoadipate + L-glutamate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.39
Bos taurus
-
bovine
-
2.6.1.39
Rattus norvegicus
-
Sprague-Dawley
-
2.6.1.39
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.39
-
Bos taurus
2.6.1.39
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.39
kidney
-
Bos taurus
-
2.6.1.39
kidney
-
Rattus norvegicus
-
2.6.1.39
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.39
0.53
-
-
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.39
4°C, partially purified enzyme in 8 mM potassium phosphate buffer, pH 7.1, 100% activity is lost when stored for 14 days
Bos taurus
2.6.1.39
4°C, partially purified enzyme in 8 mM potassium phosphate buffer, pH 7.1, 15% activity is lost when stored for 14 days
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.39
2-aminoadipate + pyruvate
-
639979
Rattus norvegicus
2-oxoadipate + L-alanine
-
-
-
r
2.6.1.39
2-aminoadipate + pyruvate
-
639979
Bos taurus
2-oxoadipate + L-alanine
-
-
-
r
2.6.1.39
3,5-di-iodotyrosine + 2-oxoglutarate
-
639979
Rattus norvegicus
3,5-diiodophenylpyruvate + L-glutamate
-
-
-
r
2.6.1.39
3,5-di-iodotyrosine + 2-oxoglutarate
-
639979
Bos taurus
3,5-diiodophenylpyruvate + L-glutamate
-
-
-
r
2.6.1.39
DL-2-aminopimelic acid + 2-oxoglutarate
-
639979
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
DL-2-aminopimelic acid + 2-oxoglutarate
-
639979
Bos taurus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
kynurenine + 2-oxoglutarate
-
639979
Rattus norvegicus
kynurenic acid + L-glutamate
-
-
-
ir
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
-
639979
Rattus norvegicus
2-oxoadipate + L-glutamate
-
639979
Rattus norvegicus
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
-
639979
Bos taurus
2-oxoadipate + L-glutamate
-
639979
Bos taurus
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
lysine catabolic pathway
639979
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
lysine catabolic pathway
639979
Bos taurus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-histidine + 2-oxoglutarate
-
639979
Rattus norvegicus
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
-
-
r
2.6.1.39
L-histidine + 2-oxoglutarate
-
639979
Bos taurus
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
-
-
r
2.6.1.39
L-histidine + 2-oxoglutarate
-
639979
Rattus norvegicus Sprague-Dawley
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
-
-
r
2.6.1.39
L-histidine + pyruvate
-
639979
Rattus norvegicus
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
-
-
r
2.6.1.39
L-histidine + pyruvate
-
639979
Bos taurus
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
-
-
r
2.6.1.39
L-histidine + pyruvate
-
639979
Rattus norvegicus Sprague-Dawley
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
-
-
r
2.6.1.39
L-norleucine + 2-oxoglutarate
-
639979
Rattus norvegicus
2-oxohexanoate + L-glutamate
-
-
-
r
2.6.1.39
L-norleucine + 2-oxoglutarate
-
639979
Bos taurus
2-oxohexanoate + L-glutamate
-
-
-
r
2.6.1.39
L-norleucine + 2-oxoglutarate
-
639979
Rattus norvegicus Sprague-Dawley
2-oxohexanoate + L-glutamate
-
-
-
r
2.6.1.39
additional information
only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor
639979
Rattus norvegicus
?
-
-
-
-
2.6.1.39
additional information
only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor
639979
Bos taurus
?
-
-
-
-
2.6.1.39
additional information
no kynurenine aminotransferase activity
639979
Bos taurus
?
-
-
-
-
2.6.1.39
additional information
2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein
639979
Rattus norvegicus
?
-
-
-
-
2.6.1.39
additional information
only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor
639979
Rattus norvegicus Sprague-Dawley
?
-
-
-
-
2.6.1.39
additional information
2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein
639979
Rattus norvegicus Sprague-Dawley
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.39
dimer
2 * 41000, SDS-PAGE
Bos taurus
2.6.1.39
dimer
2 * 41000, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.39
7
-
-
Bos taurus
2.6.1.39
7
-
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.39
pyridoxal 5'-phosphate
-
Bos taurus
2.6.1.39
pyridoxal 5'-phosphate
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.39
pyridoxal 5'-phosphate
-
Bos taurus
2.6.1.39
pyridoxal 5'-phosphate
-
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.6.1.39
0.1 mM dithiothreitol improves the stability of the enzyme by 50%
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.39
3-methylglutaric acid
6 mM, 2% inhibition
Bos taurus
2.6.1.39
3-methylglutaric acid
6 mM, 38% inhibition
Rattus norvegicus
2.6.1.39
adipic acid
6 mM, 48% inhibition
Bos taurus
2.6.1.39
adipic acid
6 mM, 60% inhibition
Rattus norvegicus
2.6.1.39
azelaic acid
6 mM, 36% inhibition
Bos taurus
2.6.1.39
azelaic acid
6 mM, 38% inhibition
Rattus norvegicus
2.6.1.39
Decanoic acid
6 mM, 50% inhibition
Bos taurus
2.6.1.39
Decanoic acid
6 mM, 45% inhibition
Rattus norvegicus
2.6.1.39
Diethylglutaric acid
6 mM, 20% inhibition
Bos taurus
2.6.1.39
Diethylglutaric acid
6 mM, 20% inhibition
Rattus norvegicus
2.6.1.39
dimethylglutaric acid
6 mM, 15% inhibition
Bos taurus
2.6.1.39
dimethylglutaric acid
6 mM, 42% inhibition
Rattus norvegicus
2.6.1.39
Glutaric acid
6 mM, 5% inhibition
Rattus norvegicus
2.6.1.39
Kynurenic acid
1 mM, 30% inhibition
Bos taurus
2.6.1.39
Kynurenic acid
1 mM, 40% inhibition
Rattus norvegicus
2.6.1.39
additional information
oxaloacetic acid is no inhibitor
Bos taurus
2.6.1.39
additional information
oxaloacetic acid is no inhibitor
Rattus norvegicus
2.6.1.39
pimelic acid
6 mM, 28% inhibition
Bos taurus
2.6.1.39
pimelic acid
6 mM, 32% inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.39
0.5
-
2-oxoadipic acid
pH 7.0, 37°C
Bos taurus
2.6.1.39
0.5
-
2-oxoadipic acid
pH 7.0, 37°C
Rattus norvegicus
2.6.1.39
4.6
-
L-Glutamic acid
pH 7.0, 37°C
Bos taurus
2.6.1.39
4.6
-
L-Glutamic acid
pH 7.0, 37°C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.39
cytoplasm
-
Bos taurus
5737
-
2.6.1.39
cytoplasm
-
Rattus norvegicus
5737
-
2.6.1.39
mitochondrion
-
Bos taurus
5739
-
2.6.1.39
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.39
41000
-
2 * 41000, SDS-PAGE
Bos taurus
2.6.1.39
41000
-
2 * 41000, SDS-PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
Rattus norvegicus
lysine catabolic pathway
2-oxoadipate + L-glutamate
-
-
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
Bos taurus
lysine catabolic pathway
2-oxoadipate + L-glutamate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.39
-
Bos taurus
2.6.1.39
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.39
kidney
-
Bos taurus
-
2.6.1.39
kidney
-
Rattus norvegicus
-
2.6.1.39
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.39
0.53
-
-
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.39
4°C, partially purified enzyme in 8 mM potassium phosphate buffer, pH 7.1, 100% activity is lost when stored for 14 days
Bos taurus
2.6.1.39
4°C, partially purified enzyme in 8 mM potassium phosphate buffer, pH 7.1, 15% activity is lost when stored for 14 days
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.39
2-aminoadipate + pyruvate
-
639979
Rattus norvegicus
2-oxoadipate + L-alanine
-
-
-
r
2.6.1.39
2-aminoadipate + pyruvate
-
639979
Bos taurus
2-oxoadipate + L-alanine
-
-
-
r
2.6.1.39
3,5-di-iodotyrosine + 2-oxoglutarate
-
639979
Rattus norvegicus
3,5-diiodophenylpyruvate + L-glutamate
-
-
-
r
2.6.1.39
3,5-di-iodotyrosine + 2-oxoglutarate
-
639979
Bos taurus
3,5-diiodophenylpyruvate + L-glutamate
-
-
-
r
2.6.1.39
DL-2-aminopimelic acid + 2-oxoglutarate
-
639979
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
DL-2-aminopimelic acid + 2-oxoglutarate
-
639979
Bos taurus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
kynurenine + 2-oxoglutarate
-
639979
Rattus norvegicus
kynurenic acid + L-glutamate
-
-
-
ir
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
-
639979
Rattus norvegicus
2-oxoadipate + L-glutamate
-
639979
Rattus norvegicus
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
-
639979
Bos taurus
2-oxoadipate + L-glutamate
-
639979
Bos taurus
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
lysine catabolic pathway
639979
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
lysine catabolic pathway
639979
Bos taurus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-histidine + 2-oxoglutarate
-
639979
Rattus norvegicus
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
-
-
r
2.6.1.39
L-histidine + 2-oxoglutarate
-
639979
Bos taurus
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
-
-
r
2.6.1.39
L-histidine + 2-oxoglutarate
-
639979
Rattus norvegicus Sprague-Dawley
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
-
-
r
2.6.1.39
L-histidine + pyruvate
-
639979
Rattus norvegicus
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
-
-
r
2.6.1.39
L-histidine + pyruvate
-
639979
Bos taurus
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
-
-
r
2.6.1.39
L-histidine + pyruvate
-
639979
Rattus norvegicus Sprague-Dawley
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
-
-
r
2.6.1.39
L-norleucine + 2-oxoglutarate
-
639979
Rattus norvegicus
2-oxohexanoate + L-glutamate
-
-
-
r
2.6.1.39
L-norleucine + 2-oxoglutarate
-
639979
Bos taurus
2-oxohexanoate + L-glutamate
-
-
-
r
2.6.1.39
L-norleucine + 2-oxoglutarate
-
639979
Rattus norvegicus Sprague-Dawley
2-oxohexanoate + L-glutamate
-
-
-
r
2.6.1.39
additional information
only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor
639979
Rattus norvegicus
?
-
-
-
-
2.6.1.39
additional information
only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor
639979
Bos taurus
?
-
-
-
-
2.6.1.39
additional information
no kynurenine aminotransferase activity
639979
Bos taurus
?
-
-
-
-
2.6.1.39
additional information
2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein
639979
Rattus norvegicus
?
-
-
-
-
2.6.1.39
additional information
only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor
639979
Rattus norvegicus Sprague-Dawley
?
-
-
-
-
2.6.1.39
additional information
2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein
639979
Rattus norvegicus Sprague-Dawley
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.39
dimer
2 * 41000, SDS-PAGE
Bos taurus
2.6.1.39
dimer
2 * 41000, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.39
7
-
-
Bos taurus
2.6.1.39
7
-
-
Rattus norvegicus