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Literature summary extracted from

  • Deshmukh, D.R.; Mungre, S.M.
    Purification and properties of 2-aminoadipate: 2-oxoglutarate aminotransferase from bovine kidney (1989), Biochem. J., 261, 761-768.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.6.1.39 0.1 mM dithiothreitol improves the stability of the enzyme by 50% Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.39 3-methylglutaric acid 6 mM, 2% inhibition Bos taurus
2.6.1.39 3-methylglutaric acid 6 mM, 38% inhibition Rattus norvegicus
2.6.1.39 adipic acid 6 mM, 48% inhibition Bos taurus
2.6.1.39 adipic acid 6 mM, 60% inhibition Rattus norvegicus
2.6.1.39 azelaic acid 6 mM, 36% inhibition Bos taurus
2.6.1.39 azelaic acid 6 mM, 38% inhibition Rattus norvegicus
2.6.1.39 Decanoic acid 6 mM, 50% inhibition Bos taurus
2.6.1.39 Decanoic acid 6 mM, 45% inhibition Rattus norvegicus
2.6.1.39 Diethylglutaric acid 6 mM, 20% inhibition Bos taurus
2.6.1.39 Diethylglutaric acid 6 mM, 20% inhibition Rattus norvegicus
2.6.1.39 dimethylglutaric acid 6 mM, 15% inhibition Bos taurus
2.6.1.39 dimethylglutaric acid 6 mM, 42% inhibition Rattus norvegicus
2.6.1.39 Glutaric acid 6 mM, 5% inhibition Rattus norvegicus
2.6.1.39 Kynurenic acid 1 mM, 30% inhibition Bos taurus
2.6.1.39 Kynurenic acid 1 mM, 40% inhibition Rattus norvegicus
2.6.1.39 additional information oxaloacetic acid is no inhibitor Bos taurus
2.6.1.39 additional information oxaloacetic acid is no inhibitor Rattus norvegicus
2.6.1.39 pimelic acid 6 mM, 28% inhibition Bos taurus
2.6.1.39 pimelic acid 6 mM, 32% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.39 0.5
-
 2-oxoadipic acid pH 7.0, 37°C Rattus norvegicus
2.6.1.39 0.5
-
 2-oxoadipic acid pH 7.0, 37°C Bos taurus
2.6.1.39 4.6
-
 L-Glutamic acid pH 7.0, 37°C Rattus norvegicus
2.6.1.39 4.6
-
 L-Glutamic acid pH 7.0, 37°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.39 cytoplasm
-
 Rattus norvegicus 5737
-
2.6.1.39 cytoplasm
-
 Bos taurus 5737
-
2.6.1.39 mitochondrion
-
 Rattus norvegicus 5739
-
2.6.1.39 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.39 41000
-
 2 * 41000, SDS-PAGE Rattus norvegicus
2.6.1.39 41000
-
 2 * 41000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate Rattus norvegicus lysine catabolic pathway 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate Bos taurus lysine catabolic pathway 2-oxoadipate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.39 Bos taurus
-
 bovine
-
2.6.1.39 Rattus norvegicus
-
 Sprague-Dawley
-
2.6.1.39 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.39
-
 Rattus norvegicus
2.6.1.39
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.39 kidney
-
 Rattus norvegicus
-
2.6.1.39 kidney
-
 Bos taurus
-
2.6.1.39 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.39 0.53
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.6.1.39 4°C, partially purified enzyme in 8 mM potassium phosphate buffer, pH 7.1, 100% activity is lost when stored for 14 days Bos taurus
2.6.1.39 4°C, partially purified enzyme in 8 mM potassium phosphate buffer, pH 7.1, 15% activity is lost when stored for 14 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.39 2-aminoadipate + pyruvate
-
 Rattus norvegicus 2-oxoadipate + L-alanine
-
 r
2.6.1.39 2-aminoadipate + pyruvate
-
 Bos taurus 2-oxoadipate + L-alanine
-
 r
2.6.1.39 3,5-di-iodotyrosine + 2-oxoglutarate
-
 Rattus norvegicus 3,5-diiodophenylpyruvate + L-glutamate
-
 r
2.6.1.39 3,5-di-iodotyrosine + 2-oxoglutarate
-
 Bos taurus 3,5-diiodophenylpyruvate + L-glutamate
-
 r
2.6.1.39 DL-2-aminopimelic acid + 2-oxoglutarate
-
 Rattus norvegicus 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 DL-2-aminopimelic acid + 2-oxoglutarate
-
 Bos taurus 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 kynurenine + 2-oxoglutarate
-
 Rattus norvegicus kynurenic acid + L-glutamate
-
 ir
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate
-
 Rattus norvegicus 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate
-
 Bos taurus 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate lysine catabolic pathway Rattus norvegicus 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate lysine catabolic pathway Bos taurus 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 L-histidine + 2-oxoglutarate
-
 Rattus norvegicus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.39 L-histidine + 2-oxoglutarate
-
 Bos taurus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.39 L-histidine + 2-oxoglutarate
-
 Rattus norvegicus Sprague-Dawley 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.39 L-histidine + pyruvate
-
 Rattus norvegicus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 r
2.6.1.39 L-histidine + pyruvate
-
 Bos taurus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 r
2.6.1.39 L-histidine + pyruvate
-
 Rattus norvegicus Sprague-Dawley 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 r
2.6.1.39 L-norleucine + 2-oxoglutarate
-
 Rattus norvegicus 2-oxohexanoate + L-glutamate
-
 r
2.6.1.39 L-norleucine + 2-oxoglutarate
-
 Bos taurus 2-oxohexanoate + L-glutamate
-
 r
2.6.1.39 L-norleucine + 2-oxoglutarate
-
 Rattus norvegicus Sprague-Dawley 2-oxohexanoate + L-glutamate
-
 r
2.6.1.39 additional information only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor Rattus norvegicus ?
-
 ?
2.6.1.39 additional information only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor Bos taurus ?
-
 ?
2.6.1.39 additional information no kynurenine aminotransferase activity Bos taurus ?
-
 ?
2.6.1.39 additional information 2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein Rattus norvegicus ?
-
 ?
2.6.1.39 additional information only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor Rattus norvegicus Sprague-Dawley ?
-
 ?
2.6.1.39 additional information 2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein Rattus norvegicus Sprague-Dawley ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.39 dimer 2 * 41000, SDS-PAGE Rattus norvegicus
2.6.1.39 dimer 2 * 41000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.39 AadAT
-
 Rattus norvegicus
2.6.1.39 AadAT
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.39 7
-
-
 Rattus norvegicus
2.6.1.39 7
-
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.39 pyridoxal 5'-phosphate
-
 Rattus norvegicus
2.6.1.39 pyridoxal 5'-phosphate
-
 Bos taurus