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Literature summary extracted from

  • Soffer, R.L.; Hechtman, P.; Savage, M.
    L-Triiodothyronine aminotransferase (1973), J. Biol. Chem., 248, 1224-1230.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.26 2-mercaptoethanol absolutely required Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.26 p-hydroxymercuribenzoate complete inhibition at 0.1 mM Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.26 0.034
-
 L-triiodothyronine pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 0.2
-
 2-oxoglutarate cosubstrate triiodothyronine, pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 0.2
-
 2-oxoglutarate cosubstrate L-thyroxine, pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 0.9
-
 3,5-diiodo-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 2
-
 pyruvate cosubstrate L-triiodothyronine, pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 2
-
 2-oxoglutarate cosubstrate 3,5-dinitro-L-tyrosine, pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 2.12
-
 L-thyronine pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 3.8
-
 3-iodo-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 4.4
-
 3,5-Dinitro-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.26 cytosol
-
 Oryctolagus cuniculus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.26 48000
-
 2 * 48000, SDS-PAGE Oryctolagus cuniculus
2.6.1.26 95000
-
 glycerol density gradient centrifugation Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.26 L-3,5,3'-triiodothyronine + 2-oxoglutarate Oryctolagus cuniculus
-
 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate i.e. 3,5,3'-triiodothyropyruvate + L-Glu r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.26 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.26
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.26 brain low activity Oryctolagus cuniculus
-
2.6.1.26 heart low activity Oryctolagus cuniculus
-
2.6.1.26 kidney
-
 Oryctolagus cuniculus
-
2.6.1.26 liver
-
 Oryctolagus cuniculus
-
2.6.1.26 lung low activity Oryctolagus cuniculus
-
2.6.1.26 additional information not in serum Oryctolagus cuniculus
-
2.6.1.26 skeletal muscle low activity Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.26 0.013
-
 purified enzyme, substrates L-3,5,3'-triiodothyronine + 2-oxoglutarate Oryctolagus cuniculus
2.6.1.26 15
-
 purified enzyme, substrates 3,5-dinitro-L-tyrosine + 2-oxoglutarate Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.6.1.26 -20°C, 30% loss of activity per month Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.26 3,5-dibromo-L-tyrosine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-(3,5-dibromo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.26 3,5-diiodo-D-tyrosine + 2-oxoglutarate very low activity Oryctolagus cuniculus 3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.26 3,5-diiodo-L-tyrosine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.26 3,5-dinitro-L-tyrosine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-(3,5-dinitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate i.e. 3,5-dinitro-p-hydroxyphenylpyruvic acid + Glu r
2.6.1.26 3-amino-L-tyrosine + 2-oxoglutarate very low activity Oryctolagus cuniculus 3-(3-amino-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.26 3-iodo-L-tyrosine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-(3-iodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.26 3-nitro-L-tyrosine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-(3-nitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.26 L-3,5,3'-triiodothyronine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate i.e. 3,5,3'-triiodothyropyruvate + L-Glu r
2.6.1.26 L-3,5,3'-triiodothyronine + oxaloacetate
-
 Oryctolagus cuniculus 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + glycine i.e. 3,5,3'-triiodothyrophenylpyruvate + Gly r
2.6.1.26 L-3,5,3'-triiodothyronine + pyruvate
-
 Oryctolagus cuniculus 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-alanine i.e. 3,5,3'-triiodothyrophenylpyruvate + L-Ala r
2.6.1.26 L-phenylalanine + 2-oxoglutarate low activtiy Oryctolagus cuniculus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.26 L-thyronine + 2-oxoglutarate
-
 Oryctolagus cuniculus ?
-
 ?
2.6.1.26 L-thyroxine + 2-oxoglutarate
-
 Oryctolagus cuniculus 3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
-
 r
2.6.1.26 L-tryptophan + 2-oxoglutarate low activtiy Oryctolagus cuniculus 3-indole-2-oxopropanoate + L-glutamate
-
 r
2.6.1.26 L-tyrosine + 2-oxoglutarate low activtiy Oryctolagus cuniculus 2-oxo-3-phenylpropanoate + L-glutamate
-
 r
2.6.1.26 additional information specific for 2-oxoglutarate Oryctolagus cuniculus ?
-
 ?
2.6.1.26 additional information pyruvate and oxalacetate are ineffective with 3,5-dinitro-L-tyrosine and only partially effective with iodinated tyrosines Oryctolagus cuniculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.26 dimer 2 * 48000, SDS-PAGE Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.26 0.0233
-
 L-thyroxine pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 0.0317
-
 L-triiodothyronine pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 1.1
-
 3,5-diiodo-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 3.68
-
 3-iodo-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 6.6
-
 3,5-diiodo-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus
2.6.1.26 39.9
-
 3,5-Dinitro-L-tyrosine pH 7.8, 37°C Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.26 7.4 8.6 broad optimum Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.26 7 8.6 pH 7.0: 30% of maximal activity with L-triiodothyronine, 15% of maximal activity with 3,5-dinitro-L-tyrosine, pH 7.4-8.6: maximal activity with triiodothyronine and 3,5-dinitro-L-tyrosine Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.26 pyridoxal 5'-phosphate partial requirement Oryctolagus cuniculus