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Literature summary extracted from

  • Goldstone, A.; Adams, E.
    Metabolism of gamma-hydroxyglutamic acid. I. Conversion to alpha-hydroxy-gamma-ketoglutarate by purified glutamic-aspartic transaminase of rat liver (1962), J. Biol. Chem., 237, 3476-3485.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.23 hydroxylamine 70% inhibition at 1 mM Rattus norvegicus
2.6.1.23 L-glutarate 63% inhibition at 0.5 mM Rattus norvegicus
2.6.1.23 Maleate 65% inhibition at 5 mM Rattus norvegicus
2.6.1.23 N-ethylmaleimide 50% inhibition at 5 mM Rattus norvegicus
2.6.1.23 p-chloromercuribenzoate 77% inhibition at 0.5 mM Rattus norvegicus
2.6.1.23 phenylhydrazine 84% inhibition at 1 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.23 0.41
-
 2-oxoglutarate pH 8.1, 37°C Rattus norvegicus
2.6.1.23 33
-
 L-glutamate pH 8.1, 37°C Rattus norvegicus
2.6.1.23 240
-
 L-erythro-4-hydroxyglutamate pH 8.1, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.23 cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.23 4-hydroxy-L-glutamate + oxo acid Rattus norvegicus metabolism of 4-hydroxy-L-glutamate in rat liver 4-hydroxy-2-oxoglutarate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.23 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.23 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.23 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.23 0.49
-
 partially purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.23 4-hydroxy-L-glutamate + 2-oxoglutarate enzyme utilises erythro-L-4-hydroxyglutamate and threo-L-4-hydroxyglutamate to the same extent, but no D-isomers Rattus norvegicus 4-hydroxy-2-oxoglutarate + L-glutamate
-
 r
2.6.1.23 4-hydroxy-L-glutamate + oxaloacetate
-
 Rattus norvegicus 4-hydroxy-2-oxoglutarate + L-aspartate
-
 r
2.6.1.23 4-hydroxy-L-glutamate + oxo acid metabolism of 4-hydroxy-L-glutamate in rat liver Rattus norvegicus 4-hydroxy-2-oxoglutarate + L-glutamate
-
 r
2.6.1.23 L-erythro-4-hydroxyglutamate + 2-oxoglutarate
-
 Rattus norvegicus D-4-hydroxy-2-oxoglutarate + L-glutamate
-
 ?
2.6.1.23 additional information substrate stereospecificity Rattus norvegicus ?
-
 ?
2.6.1.23 additional information pyruvate is a poor substrate Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.23 More may be identical with EC 2.6.1.1 aspartate transaminase Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.23 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.23 8.2
-
 with erythro-L-hydroxyglutamic acid Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.23 6.7 9.5 pH 6.7: about 45% of activity maximum, pH 9.5: about 55% of activity maximum Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.23 additional information no cofactor requirement Rattus norvegicus