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Literature summary extracted from

  • Chmara, H.; Zähner, H.
    The inactivation of glucosamine synthetase from bacteria by anticapsin, the C-terminal epoxyamino acid of the antibiotic tetaine (1984), Biochim. Biophys. Acta, 787, 45-52.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.16 1,1'-dithiodiformamidine irreversible inhibition Bacillus thuringiensis
2.6.1.16 1,1'-dithiodiformamidine irreversible inhibition Escherichia coli
2.6.1.16 1,1'-dithiodiformamidine irreversible inhibition Paenarthrobacter aurescens
2.6.1.16 1,1'-dithiodiformamidine irreversible inhibition Pseudomonas aeruginosa
2.6.1.16 5,5'-dithionitrobenzoic acid irreversible inhibition Bacillus thuringiensis
2.6.1.16 5,5'-dithionitrobenzoic acid irreversible inhibition Escherichia coli
2.6.1.16 5,5'-dithionitrobenzoic acid irreversible inhibition Paenarthrobacter aurescens
2.6.1.16 5,5'-dithionitrobenzoic acid irreversible inhibition Pseudomonas aeruginosa
2.6.1.16 6,6'-Dithiodinicotinic acid irreversible inhibition Bacillus thuringiensis
2.6.1.16 6,6'-Dithiodinicotinic acid irreversible inhibition Escherichia coli
2.6.1.16 6,6'-Dithiodinicotinic acid irreversible inhibition Paenarthrobacter aurescens
2.6.1.16 6,6'-Dithiodinicotinic acid irreversible inhibition Pseudomonas aeruginosa
2.6.1.16 anticapsin L-glutamine protects, irreversible inhibition Bacillus thuringiensis
2.6.1.16 anticapsin L-glutamine protects, irreversible inhibition Escherichia coli
2.6.1.16 anticapsin L-glutamine protects, irreversible inhibition Paenarthrobacter aurescens
2.6.1.16 anticapsin L-glutamine protects, irreversible inhibition Pseudomonas aeruginosa
2.6.1.16 iodoacetamide irreversible inhibition Bacillus thuringiensis
2.6.1.16 iodoacetamide irreversible inhibition Escherichia coli
2.6.1.16 iodoacetamide irreversible inhibition Paenarthrobacter aurescens
2.6.1.16 iodoacetamide irreversible inhibition Pseudomonas aeruginosa
2.6.1.16 N-ethylmaleimide irreversible inhibition Bacillus thuringiensis
2.6.1.16 N-ethylmaleimide irreversible inhibition Escherichia coli
2.6.1.16 N-ethylmaleimide irreversible inhibition Paenarthrobacter aurescens
2.6.1.16 N-ethylmaleimide irreversible inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.16 0.225
-
 L-glutamine pH 7.5, 37°C Paenarthrobacter aurescens
2.6.1.16 0.4
-
 L-glutamine pH 7.5, 37°C Escherichia coli
2.6.1.16 0.6
-
 L-glutamine pH 7.5, 37°C Bacillus thuringiensis
2.6.1.16 0.85
-
 L-glutamine pH 7.5, 37°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.16 Bacillus thuringiensis
-
-
-
2.6.1.16 Escherichia coli
-
-
-
2.6.1.16 Paenarthrobacter aurescens
-
-
-
2.6.1.16 Pseudomonas aeruginosa
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.16 1.2
-
-
 Pseudomonas aeruginosa
2.6.1.16 3.6
-
-
 Bacillus thuringiensis
2.6.1.16 4
-
-
 Escherichia coli
2.6.1.16 8
-
-
 Paenarthrobacter aurescens

Storage Stability

EC Number Storage Stability Organism
2.6.1.16 5°C, potassium phosphate buffer, pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, 0.5 mM L-glutamine, 0.05 mM D-glucose 6-phosphate, 600 mM sucrose, stable for few weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Escherichia coli L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Pseudomonas aeruginosa L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Paenarthrobacter aurescens L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Bacillus thuringiensis L-glutamate + D-glucosamine 6-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.16 37
-
 assay at Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.16 0.00011
-
 anticapsin pH 7.5, 37°C Pseudomonas aeruginosa
2.6.1.16 0.00053
-
 anticapsin pH 7.5, 37°C Paenarthrobacter aurescens
2.6.1.16 0.00056
-
 anticapsin pH 7.5, 37°C Escherichia coli
2.6.1.16 0.00075
-
 anticapsin pH 7.5, 37°C Bacillus thuringiensis