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Literature summary extracted from

  • Kim, H.; Ikegami, K.; Nakaoka, M.; Yagi, M.; Shibata, H.; Sawa, Y.
    Characterization of aspartate aminotransferase from the cyanobacterium Phormidium lapideum (2003), Biosci. Biotechnol. Biochem., 67, 490-498.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.1 2-oxoglutarate stabilizes against thermal inactivation Phormidium lapideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 0.032
-
 oxaloacetate pH 8.0, 45°C Phormidium lapideum
2.6.1.1 0.2
-
 2-oxoglutarate pH 8.0, 45°C Phormidium lapideum
2.6.1.1 5
-
 L-aspartate pH 8.0, 45°C Phormidium lapideum
2.6.1.1 5.7
-
 L-glutamate pH 8.0, 45°C Phormidium lapideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 42000
-
 2 * 42000, SDS-PAGE Phormidium lapideum
2.6.1.1 85000
-
 gel filtration Phormidium lapideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Phormidium lapideum
-
 oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Phormidium lapideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 2450fold Phormidium lapideum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.1 L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Phormidium lapideum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 0.11
-
 cell extract Phormidium lapideum
2.6.1.1 221
-
 purified enzyme Phormidium lapideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxobutyrate 0.4% activity compared to 2-oxoglutarate Phormidium lapideum oxaloacetate + 2-aminobutyrate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Phormidium lapideum oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate 2-oxoglutarate is the best acceptor substrate Phormidium lapideum oxaloacetate + L-glutamate
-
 r
2.6.1.1 L-aspartate + 2-oxoglutarate 45% activity with L-aspartate compared to L-glutamate Phormidium lapideum oxaloacetate + L-glutamate
-
 r
2.6.1.1 L-aspartate + 2-oxoisocaproate 1.0% activity compared to 2-oxoglutarate Phormidium lapideum oxaloacetate + L-leucine
-
 ?
2.6.1.1 L-aspartate + phenylpyruvate no activity Phormidium lapideum oxaloacetate + L-phenylalanine
-
 ?
2.6.1.1 L-aspartate + pyruvate 1.9% activity compared to 2-oxoglutarate Phormidium lapideum oxaloacetate + L-alanine
-
 ?
2.6.1.1 L-cysteine sulfinic acid + 2-oxoglutarate 27% activity compared to L-glutamate Phormidium lapideum 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
2.6.1.1 L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor Phormidium lapideum 2-oxoglutarate + L-glutamate
-
 ?
2.6.1.1 L-methionine + 2-oxoglutarate 0.1% activity compared to L-glutamate Phormidium lapideum L-glutamate + 4-methylsulfanyl-2-oxobutyric acid
-
 ?
2.6.1.1 additional information substrate specificity Phormidium lapideum ?
-
 ?
2.6.1.1 additional information indole-3-pyruvate, p-hydroxy-phenylpyruvate, and 2-oxo-n-caproate are no substrates Phormidium lapideum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.1 dimer 2 * 42000, SDS-PAGE Phormidium lapideum

Synonyms

EC Number Synonyms Comment Organism
2.6.1.1 AspAT
-
 Phormidium lapideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.1 80
-
-
 Phormidium lapideum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.1 45 85 44% of maximum activity at 45°C, 80% of maximum activity at 85°C Phormidium lapideum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.1 additional information
-
 2-oxoglutarate protects against thermal inactivation Phormidium lapideum
2.6.1.1 75
-
-
 Phormidium lapideum
2.6.1.1 80
-
 10 min, 75% remaining activity Phormidium lapideum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.1 101
-
 L-glutamate pH 8.0, 45°C Phormidium lapideum
2.6.1.1 187
-
 L-aspartate pH 8.0, 45°C Phormidium lapideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 8
-
-
 Phormidium lapideum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.1 5 8.8
-
 Phormidium lapideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.1 pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Phormidium lapideum
2.6.1.1 pyridoxal 5'-phosphate enzyme-bound Phormidium lapideum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.1 Phormidium lapideum 2 forms differing in isoelectric point: form I has a pI at pH 4.1, form II at pH 4.2, kinetic mechanism
-
 4.1
2.6.1.1 Phormidium lapideum 2 forms differing in isoelectric point: form I has a pI at pH 4.1, form II at pH 4.2, kinetic mechanism
-
 4.2