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Literature summary extracted from

  • Yagi, T.; Sako, M.; Moriuti, S.; Shounaka, M.; Masaki, K.; Yamamoto, S.
    Purification and characterization of aspartate aminotransferase isoenzymes from rice bran (1993), Biosci. Biotechnol. Biochem., 57, 2074-2080.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.1 concentrated isoenzyme AAT-1 solution, 10 mM Tris-HCl, pH 8.0, 1 mM 2-oxoglutarate, 2 mM KOH, 0.01 pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, polyethylene glycol 6000 8% w/v, 4°C, 6 days Oryza sativa
2.6.1.1 concentrated isoenzyme AAT-2 solution, 10 mM potassium phosphate, pH 7.0, 1 mM 2-oxoglutarate, 2 mM KOH, 0.001 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, dialysis against distilled water at 4°C for 3 days Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 0.043
-
 oxaloacetate isoenzyme AAT-1 Oryza sativa
2.6.1.1 0.048
-
 oxaloacetate isoenzyme AAT-2 Oryza sativa
2.6.1.1 0.105
-
 2-oxoglutarate isoenzyme AAT-2, pH 7.8, 37°C Oryza sativa
2.6.1.1 0.11
-
 2-oxoglutarate isoenzyme AAT-1, pH 7.8, 37°C Oryza sativa
2.6.1.1 2.4
-
 L-aspartate isoenzyme AAT-1, pH 7.8, 37°C Oryza sativa
2.6.1.1 3.7
-
 L-aspartate isoenzyme AAT-2, pH 7.8, 37°C Oryza sativa
2.6.1.1 5
-
 L-tryptophan L-glutamate, isoenzyme AAT-1 Oryza sativa
2.6.1.1 13.2
-
 L-glutamate isoenzyme AAT-2 Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 44000
-
 2 * 44000, isozyme AAT-1, SDS-PAGE Oryza sativa
2.6.1.1 47000
-
 2 * 47000, AAT-2, SDS-PAGE Oryza sativa
2.6.1.1 88000
-
 gel filtration Oryza sativa
2.6.1.1 88000
-
 isozyme AAT-1 Oryza sativa
2.6.1.1 94000
-
 isozyme AAT-2, gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Oryza sativa
-
 oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Oryza sativa
-
 cv. Koganemasari
-
2.6.1.1 Oryza sativa
-
 2 isoenzymes: AAT-1, AAT-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 2 isoenzymes: AAT-1, AAT-2 Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.1 leaf
-
 Oryza sativa
-
2.6.1.1 root
-
 Oryza sativa
-
2.6.1.1 seed coat bran Oryza sativa
-
2.6.1.1 stem
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 additional information
-
-
 Oryza sativa
2.6.1.1 390
-
 purified isozyme AAT-1 Oryza sativa
2.6.1.1 450
-
 purified isozyme AAT-2 Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Oryza sativa oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Oryza sativa oxaloacetate + L-glutamate
-
 r
2.6.1.1 L-cysteine sulfinic acid + 2-oxoglutarate 63% of the activity with aspartate Oryza sativa 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
2.6.1.1 L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor Oryza sativa 2-oxoglutarate + L-glutamate
-
 ?
2.6.1.1 L-phenylalanine + 2-oxoglutarate
-
 Oryza sativa 2-oxo-3-phenylpropionic acid + L-glutamate i.e. phenylpyruvate ?
2.6.1.1 L-tryptophan + 2-oxoglutarate
-
 Oryza sativa 3-indole-2-oxopropionic acid + L-glutamate
-
 ?
2.6.1.1 additional information substrate specificity Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.1 dimer 2 * 44000, isozyme AAT-1, SDS-PAGE Oryza sativa
2.6.1.1 dimer 2 * 47000, AAT-2, SDS-PAGE Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 7 8 forward reaction, isoenzymes AAT-1 and AAT-2 Oryza sativa
2.6.1.1 8 8.5 reverse reaction, isoenzyme AAT-1 Oryza sativa
2.6.1.1 8 8.8 reverse reaction, isoenzyme AAT-2 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.1 pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Oryza sativa
2.6.1.1 pyridoxal 5'-phosphate isozyme AAT-1: 0.87 mol per mol of subunit, isozyme AAT-2: 0.93 mol per mol of subunit Oryza sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.1 Oryza sativa isozyme AAT-2
-
 5
2.6.1.1 Oryza sativa isozyme AAT-1
-
 6.5