EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.62 | amplified by the polymerase chain reaction and cloned into T7 RNA polymerase-based expression plasmid, heterologous expression of chlG in Escherichia coli | Synechocystis sp. |
2.5.1.133 | expression in Escherichia coli | Rhodobacter capsulatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.133 | geranylgeranyl diphosphate + bacteriochlorophyllide a | Rhodobacter capsulatus | - |
geranylgeranyl-bacteriochlorophyllide a + diphosphate | - |
? | |
2.5.1.133 | additional information | Rhodobacter capsulatus | no substrate: chlorophyllide a | ? | - |
? | |
2.5.1.133 | phytyl diphosphate + bacteriochlorophyllide a | Rhodobacter capsulatus | - |
phytyl-bacteriochlorophyllide a + diphosphate | enzyme exhibits a marked preference for phytyl diphosphate over geranylgeranyl diphosphate | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.62 | Synechocystis sp. | - |
PCC 6803 | - |
2.5.1.133 | Rhodobacter capsulatus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.62 | chlorophyllide a + geranylgeranyl diphosphate | 42% of the activity with phythyl diphosphate | Synechocystis sp. | geranylgeranylchlorophyllide a + diphosphate | - |
? | |
2.5.1.62 | chlorophyllide a + phytyl diphosphate | marked preference of phytyl diphosphate as substrate over geranylgeranyl diphosphate | Synechocystis sp. | chlorophyll a + diphosphate | - |
? | |
2.5.1.62 | additional information | no activity with bacteriochlorophyllide a | Synechocystis sp. | ? | - |
? | |
2.5.1.133 | geranylgeranyl diphosphate + bacteriochlorophyllide a | - |
Rhodobacter capsulatus | geranylgeranyl-bacteriochlorophyllide a + diphosphate | - |
? | |
2.5.1.133 | additional information | no substrate: chlorophyllide a | Rhodobacter capsulatus | ? | - |
? | |
2.5.1.133 | phytyl diphosphate + bacteriochlorophyllide a | - |
Rhodobacter capsulatus | phytyl-bacteriochlorophyllide a + diphosphate | enzyme exhibits a marked preference for phytyl diphosphate over geranylgeranyl diphosphate | ? |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.133 | physiological function | heterologous expression of the bchG gene product in Escherichia coli confers bacteriochlorophyll a synthase activity. Bacteriochlorophyll a synthase utilizes bacteriochlorophyllide a, but not chlorophyllide a as a substrate | Rhodobacter capsulatus |