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Literature summary extracted from

  • Gosset, G.; Bonner, C.A.; Jensen, R.A.
    Microbial origin of plant-type 2-keto-3-deoxy-D-arabino-heptulosonate 7-phosphate synthases, exemplified by the chorismate- and tryptophan-regulated enzyme from Xanthomonas campestris (2001), J. Bacteriol., 183, 4061-4070.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.54 additional information not hysteretically activated by dithiols, e.g. DTT Xanthomonas campestris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 2 clones M1 and M2, expression in Escherichia coli strain BL21(DE3) and strain AB3248, which is defective in all endogenous isozyme forms, functional complementation of the mutant Xanthomonas campestris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 chorismate main allosteric feedback inhibitor and regulator for enzyme of class AroAII Xanthomonas campestris
2.5.1.54 EDTA strong, reversible by dialysis Xanthomonas campestris
2.5.1.54 Trp minor feedback inhibitor Xanthomonas campestris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 additional information
-
Xanthomonas campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Xanthomonas campestris
-
2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Xanthomonas campestris
-
strain ATCC 33436
-
2.5.1.54 Xanthomonas campestris
-
2 classes of enzymes: class AroAI and AroAII
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 partial, 19fold, recombinant from Escherichia coli Xanthomonas campestris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 0.8
-
recombinant clone M1 in Escherichia coli strain BL21(DE3) Xanthomonas campestris
2.5.1.54 2.4
-
partially purified native enzyme Xanthomonas campestris
2.5.1.54 3.11
-
recombinant clone M2 in Escherichia coli strain BL21(DE3) Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Xanthomonas campestris 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 More 2 distinct homology classes: AroAI and AroAII, both being found in bacteria and higher plant chloroplasts, AroAII can be devided into 4 subgroups, phylogenetic evolution Xanthomonas campestris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 52
-
-
Xanthomonas campestris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 8.2 8.5 native enzyme Xanthomonas campestris