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Literature summary extracted from

  • Liao, H.F.; Lin, L.L.; Chien, H.R.; Hsu, W.H.
    Serine 187 is a crucial residue for allosteric regulation of Corynebacterium glutamicum 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (2001), FEMS Microbiol. Lett., 194, 59-64.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.54 Phe slightly activating, mutants S187Y, S187F, S187C Corynebacterium glutamicum
2.5.1.54 Tyr mutants S187Y, S187F, S187C Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 overexpression of His-tagged wild-type and mutants in Escherichia coli Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.54 S187A site-directed mutagenesis, slightly reduced activity Corynebacterium glutamicum
2.5.1.54 S187C site-directed mutagenesis, reduced activity, activation by tyrosine and phenylalanine instead of inhibition like the wild-type Corynebacterium glutamicum
2.5.1.54 S187F site-directed mutagenesis, highly reduced activity, activation by tyrosine and phenylalanine instead of inhibition like the wild-type Corynebacterium glutamicum
2.5.1.54 S187Y site-directed mutagenesis, highly reduced activity, activation by tyrosine and phenylalanine instead of inhibition like the wild-type Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 Phe slight inhibition of the wild-type, activation of mutants Corynebacterium glutamicum
2.5.1.54 Tyr feedback inhibition; noncompetitive with respect to D-erythrose 4-phosphate, competitive with respect to phosphoenolpyruvate; Tyr-sensitive isozyme; wild-type and mutant S187A Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 additional information
-
 additional information mutants Corynebacterium glutamicum
2.5.1.54 0.0032
-
 phosphoenolpyruvate wild-type, pH 7.9, 30°C Corynebacterium glutamicum
2.5.1.54 0.018
-
 D-erythrose 4-phosphate wild-type, pH 7.9, 30°C Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Corynebacterium glutamicum
-
 Tyr-sensitive isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 recombinant His-tagged wild-type and mutants from Escherichia coli Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 additional information
-
 mutants, influence of aromatic amino acids on activity Corynebacterium glutamicum
2.5.1.54 160
-
 wild-type Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Corynebacterium glutamicum 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?