EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.54 | Phe-sensitive isozyme, wild-type and mutants, expression in Escherichia coli strain BL21(DE3) | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.54 | C61G | site-directed mutagenesis, highly reduced activity, highly increased Km for phosphoenolpyruvate, higher pH-optimum than the wild-type | Escherichia coli |
2.5.1.54 | H172G | site-directed mutagenesis, inactive | Escherichia coli |
2.5.1.54 | H207G | site-directed mutagenesis, reduced activity, increased Km values for the substrates, reduced kcat | Escherichia coli |
2.5.1.54 | H268G | site-directed mutagenesis, inactive | Escherichia coli |
2.5.1.54 | H304G | site-directed mutagenesis, reduced activity, increased Km values for the substrates, increased kcat | Escherichia coli |
2.5.1.54 | H64G | site-directed mutagenesis, reduced activity, increased Km values for the substrates, increased kcat | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.54 | diethyl dicarbonate | Phe-sensitive isozyme, pH-dependent, phosphoenolpyruvate protects wild-type and mutants H64G, H207G, H304G | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.54 | 0.009 | - |
phosphoenolpyruvate | Phe-sensitive isozyme, pH 6.8, 25°C | Escherichia coli | |
2.5.1.54 | 0.086 | - |
D-erythrose 4-phosphate | Phe-sensitive isozyme, pH 6.8, 25°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.54 | Escherichia coli | - |
phenylalanine-sensitive isozyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.54 | Phe-sensitive isozyme, recombinant wild-type from overexpressing strain and recombinant mutants | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | His268 is located in the active site | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Escherichia coli | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
ir |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 25 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.54 | 32 | - |
phosphoenolpyruvate | Phe-sensitive isozyme, pH 6.8, 25°C | Escherichia coli | |
2.5.1.54 | 32 | - |
D-erythrose 4-phosphate | Phe-sensitive isozyme, pH 6.8, 25°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 6.8 | - |
wild-type Phe-sensitive isozyme | Escherichia coli |
2.5.1.54 | 8 | - |
above, Phe-sensitive iaozyme mutant C61G | Escherichia coli |