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Literature summary extracted from

  • Howe, D.L.; Duewel, H.S.; Woodard, R.W.
    Histidine 268 in 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase plays the same role as histidine 202 in 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase (2000), J. Biol. Chem., 275, 40258-40265.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 Phe-sensitive isozyme, wild-type and mutants, expression in Escherichia coli strain BL21(DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.54 C61G site-directed mutagenesis, highly reduced activity, highly increased Km for phosphoenolpyruvate, higher pH-optimum than the wild-type Escherichia coli
2.5.1.54 H172G site-directed mutagenesis, inactive Escherichia coli
2.5.1.54 H207G site-directed mutagenesis, reduced activity, increased Km values for the substrates, reduced kcat Escherichia coli
2.5.1.54 H268G site-directed mutagenesis, inactive Escherichia coli
2.5.1.54 H304G site-directed mutagenesis, reduced activity, increased Km values for the substrates, increased kcat Escherichia coli
2.5.1.54 H64G site-directed mutagenesis, reduced activity, increased Km values for the substrates, increased kcat Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 diethyl dicarbonate Phe-sensitive isozyme, pH-dependent, phosphoenolpyruvate protects wild-type and mutants H64G, H207G, H304G Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.009
-
phosphoenolpyruvate Phe-sensitive isozyme, pH 6.8, 25°C Escherichia coli
2.5.1.54 0.086
-
D-erythrose 4-phosphate Phe-sensitive isozyme, pH 6.8, 25°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli
-
phenylalanine-sensitive isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 Phe-sensitive isozyme, recombinant wild-type from overexpressing strain and recombinant mutants Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate His268 is located in the active site Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 25
-
assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 32
-
phosphoenolpyruvate Phe-sensitive isozyme, pH 6.8, 25°C Escherichia coli
2.5.1.54 32
-
D-erythrose 4-phosphate Phe-sensitive isozyme, pH 6.8, 25°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 6.8
-
wild-type Phe-sensitive isozyme Escherichia coli
2.5.1.54 8
-
above, Phe-sensitive iaozyme mutant C61G Escherichia coli