Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rubin, J.L.; Jensen, R.A.
    Differentially regulated isoenzymes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from seedlings of Vigna radiata (1985), Plant Physiol., 79, 711-718.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.54 Chorismic acid activates isozyme DS-Mn Vigna radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 3,4-dihydroxycinnamate isoenzyme DS-Co Vigna radiata
2.5.1.54 D-erythrose 4-phosphate isoenzyme DS-Mn: substrate inhibition above 0.5 mM Vigna radiata
2.5.1.54 L-arogenate isozyme DS-Mn, competitive against D-erythrose 4-phosphate, non-competitive against phosphoenolpyruvate Vigna radiata
2.5.1.54 prephenate isozyme DS-Mn, competitive against D-erythrose 4-phosphate, non-competitive against phosphoenolpyruvate Vigna radiata
2.5.1.54 Trp L-Trp, weak Vigna radiata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Co2+ isoenzyme Ds-Co has an absolute requirement for divalent cations, Co2+ being best Vigna radiata
2.5.1.54 Mn2+ isoenzyme DS-Mn is activated 2.6fold by 0.4 mM Mn2+ Vigna radiata

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Vigna radiata
-
2 differentially regulated isozymes: DS-Mn and DS-Co
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 partial, isozyme DS-Co and isozyme DS-Mn Vigna radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.54 seedling
-
Vigna radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 0.008
-
partially purified isoenzyme DS-Mn Vigna radiata
2.5.1.54 0.21
-
partially purified isozyme DS-Co Vigna radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Vigna radiata 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7
-
isoenzyme DS-Mn Vigna radiata
2.5.1.54 8.8
-
isoenzyme DS-Co Vigna radiata