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Literature summary extracted from

  • Doong, R.L.; Gander, J.E.; Ganson, R.J.; Jensen, R.A.
    The cytosolic isoenzyme of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in Spinacia oleracea and other higher plants: extreme substrate ambiguity and other properties (1992), Physiol. Plant., 84, 351-360.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 1.95
-
 D-erythrose 4-phosphate cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
2.5.1.54 2.5
-
 D-erythrose cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
2.5.1.54 3
-
 DL-glyceraldehyde 3-phosphate cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
2.5.1.54 3.3
-
 L-glyceraldehyde cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
2.5.1.54 3.5
-
 D-glyceraldehyde cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
2.5.1.54 3.6
-
 glyoxylate cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
2.5.1.54 5.1
-
 L-erythrose cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
2.5.1.54 8.4
-
 D-threose cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
2.5.1.54 8.6
-
 glycolaldehyde cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
2.5.1.54 13.9
-
 L-threose cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.54 chloroplast chloroplastidic isoenzyme DS-Mn Spinacia oleracea 9507
-
2.5.1.54 cytosol
-
 Nicotiana sylvestris 5829
-
2.5.1.54 cytosol cytosolic isoenzyme DS-Co Spinacia oleracea 5829
-
2.5.1.54 cytosol cytosolic isoenzyme DS-Co Solanum tuberosum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Co2+
-
 Solanum tuberosum
2.5.1.54 Co2+
-
 Vigna radiata
2.5.1.54 Co2+ absolute requirement for divalent metal, 70% of the activation with Mg2+ Spinacia oleracea
2.5.1.54 Mg2+
-
 Solanum tuberosum
2.5.1.54 Mg2+ absolute requirement for divalent metal, Mg2+ is most effective Spinacia oleracea
2.5.1.54 Mn2+
-
 Solanum tuberosum
2.5.1.54 Mn2+
-
 Vigna radiata
2.5.1.54 Mn2+ absolute requirement for divalent metal, 15% of the activation with Mg2+ Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 385000 443000 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Nicotiana sylvestris
-
 cytosolic isozyme
-
2.5.1.54 Pisum sativum
-
 cytosolic isozyme Ds-Co
-
2.5.1.54 Solanum tuberosum
-
 cytosolic isozyme Ds-Co
-
2.5.1.54 Spinacia oleracea
-
 cytosolic isozyme Ds-Co
-
2.5.1.54 Vigna radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 partial Spinacia oleracea
2.5.1.54 partial Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.54 cell culture
-
 Solanum tuberosum
-
2.5.1.54 cell culture
-
 Nicotiana sylvestris
-
2.5.1.54 cell suspension culture
-
 Solanum tuberosum
-
2.5.1.54 cell suspension culture
-
 Nicotiana sylvestris
-
2.5.1.54 leaf
-
 Pisum sativum
-
2.5.1.54 seedling
-
 Spinacia oleracea
-
2.5.1.54 seedling
-
 Solanum tuberosum
-
2.5.1.54 seedling
-
 Vigna radiata
-
2.5.1.54 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 18.8
-
 partially purified cytosolic isozyme, + 10 mM Mg2+ Pisum sativum

Storage Stability

EC Number Storage Stability Organism
2.5.1.54 -80°C, isoenzyme DS-Co, stable for at least several months Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D,L-lyxose D-lyxose or L-lyxose, at 1.8% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D,L-galacto-octonate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-arabinose at 0.7% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D-gluco-octonate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-arabinose 5-phosphate at 8% of the activity with D-erythrose 4-phosphate Spinacia oleracea 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose at 93% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D-arabino-heptonate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Spinacia oleracea 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ir
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Pisum sativum 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Solanum tuberosum 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Vigna radiata 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Nicotiana sylvestris 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-glucose 6-phosphate at 0.5% of the activity with D-erythrose 4-phosphate Spinacia oleracea ?
-
 ?
2.5.1.54 phosphoenolpyruvate + D-glyceraldehyde at 176% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D-threo-hexonate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-ribulose 5-phosphate at 12% of the activity with D-erythrose 4-phosphate Spinacia oleracea ?
-
 ?
2.5.1.54 phosphoenolpyruvate + D-threose at 92% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D-xylo-heptonate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-xylose at 1% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D-ido-octonate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + DL-glyceraldehyde 3-phosphate at 142% of the activity with D-erythrose 4-phosphate Spinacia oleracea pyruvate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + glycolaldehyde at 245% of the activity with D-erythrose 4-phosphate Spinacia oleracea (S)-2-oxo-4,5-dihydroxypentanoate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + glyoxylate at 205% of the activity with D-erythrose 4-phosphate Spinacia oleracea S-4-hydroxy-2-oxo-1,5-pentanedioate + R-4-hydroxy-2-oxo-1,5-pentanedioate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + L-erythrose at 70% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-L-arabino-heptonate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + L-glyceraldehyde at 212% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-L-threo-hexonate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + L-threose at 52% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-L-xylo-heptonate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + L-xylose at 1% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-L-ido-octonate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 37
-
 assay at Spinacia oleracea
2.5.1.54 37
-
 assay at Pisum sativum
2.5.1.54 37
-
 assay at Solanum tuberosum
2.5.1.54 37
-
 assay at Nicotiana sylvestris
2.5.1.54 47 49
-
 Spinacia oleracea
2.5.1.54 47 49
-
 Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.54 37
-
 isoenzyme Ds-Co, 95% loss of activity in absence of phosphoenolpyruvate, 1.1 mM phosphoenolpyruvate almost completely protects from inactivation Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7.5 8 chloroplastic isoenzyme DS-Mn Spinacia oleracea
2.5.1.54 8.6
-
 assay at Spinacia oleracea
2.5.1.54 8.6
-
 assay at Pisum sativum
2.5.1.54 8.6
-
 assay at Solanum tuberosum
2.5.1.54 8.6
-
 assay at Nicotiana sylvestris
2.5.1.54 8.8
-
-
 Vigna radiata
2.5.1.54 9.2
-
 cytosolic isoenzyme DS-Co Spinacia oleracea