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Literature summary extracted from

  • Suzuki, N.; Sakuta, M.; Shimizu, S.
    Purification and characterization of a cytosolic isozyme of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from cultured carrot cells (1996), J. Plant Physiol., 149, 19-22.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 3.3
-
 D-erythrose 4-phosphate cytosolic isozyme, pH 8.6 Daucus carota

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.54 cytosol
-
 Daucus carota 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Co2+ stimulates Daucus carota
2.5.1.54 Mg2+ stimulates Daucus carota
2.5.1.54 Mn2+ stimulates Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 115000
-
 x * 115000, cytosolic isozyme, SDS-PAGE Daucus carota
2.5.1.54 400000
-
 gel filtration Daucus carota

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Daucus carota first enzyme of the aromatic amino acid biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Daucus carota
-
 cytosolic isozyme
-
2.5.1.54 Daucus carota
-
 cv. Kurodagosun
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 cytosolic isozyme, 79.8fold to homogeneity Daucus carota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.54 cell culture
-
 Daucus carota
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 2.3
-
 purified cytosolic isozyme Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Daucus carota 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme of the aromatic amino acid biosynthesis Daucus carota 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 ? x * 115000, cytosolic isozyme, SDS-PAGE Daucus carota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 9
-
 cytosolic isozyme Daucus carota