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Literature summary extracted from

  • Nimmo, G.A.; Coggins, J.R.
    The purification and molecular properties of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Neurospora crassa (1981), Biochem. J., 197, 427-436.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 Phe Phe-sensitive isozyme Neurospora crassa
2.5.1.54 Trp Trp-sensitive isozyme Neurospora crassa
2.5.1.54 Tyr Tyr-sensitive isozyme Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 52000
-
 4 * 52000, tryptophan-sensitive isozyme, SDS-PAGE Neurospora crassa
2.5.1.54 200000
-
 tryptophan-sensitive isozyme, sedimentation equilibrium centrifugation Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Neurospora crassa
-
-
-
2.5.1.54 Neurospora crassa
-
 Trp-, Phe- and Tyr-sensitive isoenzymes
-
2.5.1.54 Neurospora crassa 74-OR23-1A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 2350fold purification of the tryptophan sensitive isozyme, partial purification of the tyrosine- and phenylalanine-sensitive isozyme Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 0.27
-
 partially purified Tyr-sensitive isozyme Neurospora crassa
2.5.1.54 4.5
-
 partially purified Phe-sensitive isozyme Neurospora crassa
2.5.1.54 8.1
-
 purified Trp-sensitive isozyme Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Neurospora crassa 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Neurospora crassa 74-OR23-1A 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 tetramer 4 * 52000, tryptophan-sensitive isozyme, SDS-PAGE Neurospora crassa