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Literature summary extracted from

  • Whitaker, R.J.; Fiske, M.J.; Jensen, R.A.
    Pseudomonas aeruginosa possesses two novel regulatory isozymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (1982), J. Biol. Chem., 257, 12789-12794.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 chorismate competitive against phosphoenolpyruvate, noncompetitive against D-erythrose 4-phosphate; DAHP synthase-trp Pseudomonas aeruginosa
2.5.1.54 phenylpyruvate DAHP synthase-tyr Pseudomonas aeruginosa
2.5.1.54 Trp DAHP synthase-trp; non-competitive against phosphoenolpyruvate and competitive for D-erythrose 4-phosphate; Trp-sensitive isozyme Pseudomonas aeruginosa
2.5.1.54 Tyr DAHP synthase-tyr; L-Tyr; Tyr-sensitive isozyme Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.56
-
 D-erythrose 4-phosphate DAHP synthase-trp, pH 7.0, 37°C Pseudomonas aeruginosa
2.5.1.54 1
-
 phosphoenolpyruvate DAHP synthase-tyr, pH 7.0, 37°C Pseudomonas aeruginosa
2.5.1.54 1
-
 D-erythrose 4-phosphate DAHP synthase-tyr, pH 7.0, 37°C Pseudomonas aeruginosa
2.5.1.54 1.11
-
 phosphoenolpyruvate DAHP synthase-trp, pH 7.0, 37°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 137000
-
 DAHP synthase-tyr, gel filtration Pseudomonas aeruginosa
2.5.1.54 175000
-
 DAHP synthase-trp, gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Pseudomonas aeruginosa
-
 2 isoenzymes: tyrosine-sensitive DAHP synthase-tyr and tryptophane-sensitive DAHP synthase-trp
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 partially, 2 isozymes Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 0.005
-
 partially purified isozyme synthase-trp Pseudomonas aeruginosa
2.5.1.54 0.056
-
 partially purified isozyme synthase-tyr Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Pseudomonas aeruginosa 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 37
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 6.8
-
 DAHP synthase-tyr Pseudomonas aeruginosa
2.5.1.54 7
-
 assay at Pseudomonas aeruginosa
2.5.1.54 7.2
-
 DAHP synthase-trp Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.54 0.005
-
 Trp synthase-trp isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 Pseudomonas aeruginosa
2.5.1.54 0.023
-
 Tyr synthase-tyr isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 Pseudomonas aeruginosa
2.5.1.54 0.023
-
 Tyr synthase-tyr isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 Pseudomonas aeruginosa
2.5.1.54 0.04
-
 Trp synthase-trp isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 Pseudomonas aeruginosa
2.5.1.54 1.35
-
 chorismate synthase-trp isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 Pseudomonas aeruginosa
2.5.1.54 1.35
-
 phenylpyruvate synthase-tyr isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 Pseudomonas aeruginosa
2.5.1.54 2.25
-
 chorismate synthase-trp isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 Pseudomonas aeruginosa
2.5.1.54 2.55
-
 phenylpyruvate synthase-tyr isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 Pseudomonas aeruginosa